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CD4 Absolute T Cell Count (FLOW Cytometry)
MessageFor Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.
Any specimen collected on Friday and/or the weekday prior to a Holiday must be called by 2:00 pm for appropriate arrangements to be made: Contact Flow Cytometry (937-208-6687 or 1-800-686-2252 option 3, option 2).
Any specimen collected on Friday and/or the weekday prior to a Holiday must be called by 2:00 pm for appropriate arrangements to be made: Contact Flow Cytometry (937-208-6687 or 1-800-686-2252 option 3, option 2).
Test Code
CD4
Alias/See Also
74143
CPT Codes
86361
Includes
% Helper Cells (CD4) and Total Absolute Helper cells (CD4).
Preferred Specimen
Venouse Peripheral Blood: 5 mLs K3EDTA (lavender top) tube. (minimum 2 -3 mLs)
Instructions
K3EDTA: Avoid hemolysis. Gently invert 10 times.
Transport Container
K3EDTA (lavender top) tube
Transport Temperature
Room Temperature and transport without delay.
Specimen Stability
Room Temperature = 24 hours post collection
Refrigerated = unacceptable
Frozen = unacceptable
Refrigerated = unacceptable
Frozen = unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Rejected for gross hemolysis, clotted. Microcontainers; finger or heel sticks
Methodology
Flow Cytometry
Setup Schedule
Analysis performed Monday - Friday 7:00 am - 6:00 pm. Test not performed on weekends.
RFMVH
RFMVH
Report Available
1 - 2 days
Reference Range
SEX | AGE | Report Normals | Units | |
% Helper Cells (CD4) | N/M/F | 6M | 50 - 57 | % |
12M | 49 - 55 | % | ||
18M | 46 - 51 | % | ||
24M | 42 - 48 | % | ||
30M | 38 - 46 | % | ||
36M | 33 - 44 | % | ||
48M | 27 - 57 | % | ||
133Y | 32 - 61 | % | ||
Total Helper (CD4) | N/M/F | 6M | 2800 - 3900 | /MM3 |
12M | 2600 - 3500 | /MM3 | ||
18M | 2300 - 2900 | /MM3 | ||
24M | 1900 - 2500 | /MM3 | ||
30M | 1500 - 2200 | /MM3 | ||
36M | 1200 - 2000 | /MM3 | ||
48M | 560 - 2700 | /MM3 | ||
133Y | 384 - 2196 | /MM3 |
Clinical Significance
Assists in evaluating helper cell immune status in immunodeficiency diseases such as AIDS.
Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439
Last Updated: December 2, 2022