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T3 Lymphocyte Count (FLOW Cytometry)
MessageFor Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.
COLLECTION DAYS EFFECTIVE 10/24/22: MON., TUES.
(Testing performed TUES AND WEDS).
This is NOT a Thyroid function test. This test is usually performed on transplant patients. Any specimen collected on Friday and/or the weekday prior to a Holiday must be called by 2:00 pm for appropriate arrangements to be made: Contact Flow Cytometry (937-208-6687 or 1-800-686-2252 option 3, option2).
COLLECTION DAYS EFFECTIVE 10/24/22: MON., TUES.
(Testing performed TUES AND WEDS).
This is NOT a Thyroid function test. This test is usually performed on transplant patients. Any specimen collected on Friday and/or the weekday prior to a Holiday must be called by 2:00 pm for appropriate arrangements to be made: Contact Flow Cytometry (937-208-6687 or 1-800-686-2252 option 3, option2).
Test Code
CD3
Alias/See Also
71049
CPT Codes
86359
Includes
% T cells (CD3) and Total Absolute T cells (CD3).
Preferred Specimen
Venous Peripheral Blood: 5 mL K3EDTA (lavender top) tube with the total WBC and percent lymph count or 5 mL Sodium Heparin (dark green top) tube and an K3EDTA (lavender top) tube with the total WBC and percent lymph count.
Minimum Volume
(minimum 2 - 3 mLs)
Instructions
K3EDTA; Avoid Hemolysis, gently invert 10 times.
Transport Container
K3EDTA (lavender top) tube or Sodium Heparin (dark green top) tube.
Transport Temperature
Room Temperature and transport without delay.
Specimen Stability
Room Temperature = 24 hours post collection
Refrigerated = unacceptable
Frozen = unacceptable
Refrigerated = unacceptable
Frozen = unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Rejected for gross hemolysis, clotted. Microcontainers; finger and heel sticks
Methodology
Flow Cytometry
Setup Schedule
Analysis performed Monday - Friday 7:00 am - 6:00 pm and on weekends for transplant patients.
RFMVH
RFMVH
Report Available
1 day
Reference Range
| SEX | AGE | Report Normals | Units | |
| % T Cells (CD3) | N/M/F | 48M | 55 - 82 | % |
| 133Y | 60 - 90 | % | ||
| Total T Cells (CD3) | N/M/F | 6M | 3500 - 5000 | /MM3 |
| 12M | 3400 - 4600 | /MM3 | ||
| 18M | 3200 - 3900 | /MM3 | ||
| 24M | 2800 - 3500 | /MM3 | ||
| 30M | 2300 - 3300 | /MM3 | ||
| 36M | 1900 - 3100 | /MM3 | ||
| 48M | 1000 - 3900 | /MM3 | ||
| 133Y | 720 - 3240 | /MM3 |
Clinical Significance
To evaluate the absolute value of T lymphocytes in transplant patients.
Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439
Last Updated: October 24, 2022
