Culture, Blood

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

Test Code

Alias/See Also
Blood Culture;
Brucella, Blood Culture

CPT Codes
87040***When a culture is positive there will be a reflex for each identification and susceptibility performed

Preferred Specimen
Number of cultures: 2 - 3 separate sets (each set consisting of 2 10mL bottles) should be collected (collection of only a single blood culture set will miss approximately 20% of the cases detected with 3 culture sets). Adults - 20 mL (2 -10 mL bottle)

Blood (venous or arterial)
1. Prepare the site using an iodophor or betadine.  Similarly, wipe the rubber diaphragm on the blood culture bottles.
2. Allow the disinfectant to dry completely.
3. Collect the blood and inoculate both bottles and split evenly between both bottles.
4. Submit both bottles in a ziplock bag in case of leakage.
5. DO NOT INCUBATE prior to submission.  Submit at room temperature.

Transport Container
Blood Culture Bottle Set (includes both aerobic and anaerobic)

Transport Temperature
Room Temperature

Specimen Stability
Room Temperature = 1 day
Refrigerated = unacceptable
Frozen = unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
If received refrigerated or frozen

Inoculation to Standard Bacteriological Media with biochemical identification of isolated organisms.

Setup Schedule
Sunday - Saturday

Report Available

Clinical Significance
Bacterial sepsis constitutes one of the most serious infectious diseases. The detection of microorganisms in a patient's blood has importance in the diagnosis and prognosis of endocarditis, septicemia, or chronic bacteremia.

Performing Laboratory
Compunet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439

Last Updated: December 7, 2021

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.