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T-Spot TB
MessageFor Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.
Specimen should arrive at CompuNet's main lab before midnight tansport at room temperature.
Specimen should arrive at CompuNet's main lab before midnight tansport at room temperature.
Test Code
TSPOT
Alias/See Also
75050;
T Spot AB
T Spot AB
CPT Codes
86481
Preferred Specimen
1 Sodium Heparin (dark green top) tube. Adults and children over 10 years: 6 mL Whole Blood (minimum). Children 2 - 9 years of age: 4 mL Whole Blood (minimum). Children up to 2 years of age: 2 mL Whole Blood (minimum).
Instructions
Avoid hemolysis. Store at room temperature. DO NOT refrigerate.
Specimen should arrive at CompuNet's main lab before midnight (on the collection date).
Specimen should arrive at CompuNet's main lab before midnight (on the collection date).
Transport Container
Sodium Heparin (dark green top) tube
Transport Temperature
Room Temperature
Specimen should arrive at CompuNet's main lab before midnight (on the collection date).
Specimen should arrive at CompuNet's main lab before midnight (on the collection date).
Specimen Stability
Room Temperature = 30 hours
Refrigerated = unacceptable
Frozen = unacceptable
Refrigerated = unacceptable
Frozen = unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Rejected if received refrigerated or frozen. Rejected for low white cell count.
Methodology
Lymphocyte Stimulation with Immunoassay
Setup Schedule
Monday - Sunday
BS
BS
Report Available
3 days
Clinical Significance
The T-Spot test is based upon a cell-mediated immune response in TB-infected individuals. The T cells of the patient are stimulated with Mycobacterium tuberculosis antigens. If the patient has previously been exposed to Mycobacterium tuberculosis, the exposure to these antigens in the T-Spot assay stimulate the patient's T cells to secrete the cytokine interferon-γ. The presence of the interferon-γ is then detected using an EIA method. According to the CDC, this assay may be used as a substitute for a TB skin test. It is more specific than the TB skin test, will detect latent TB, and a positive result is obtained in about 85% of the patients with active TB.
Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439
Last Updated: November 23, 2021