Quanti-FERON® TB-Gold [74451]

Message
Send patients to PSC for collection. Quanti-FERON® TB-Gold in-tube collection tubes required.

Test Code
QTF

Alias/See Also
74451

CPT Codes
86480

Preferred Specimen
QuantiFERON In-Tube collection tubes required.
In-Tube collection tubes are a set of 3 or 4 tubes that are used specifically for collection for Quanti-FERON® TB-Gold testing.

Instructions
NOTE: Outpatients from a doctor's office must go to a PSC to have the sample collected.
Hospitalized patients will be drawn by the phlebotomy staff at the hospital.

QuantiFERON(TM)In-Tube collection tubes REQUIRED. In-Tube collection tubes are a set of 3 or 4 tubes that are used specifically for collection for QuantiFERON(TM) testing.

Draw blood in all 3 tubes, (or 4 tubes if collecting QTF Plus) shake vigorously (until frothy), and send to laboratory at room temperature (DO NOT REFRIGERATE). Must be processed within 16 hours of collection.
DO NOT SPIN THE TUBES

Transport Container
Quanti-FERON® TB-Gold in-tube collection tubes

Transport Temperature
Room Temperature
     For specimen integrity in the lock box click here

Specimen Stability
Room Temperature = 16 hours
Refrigerated = unacceptable
Frozen = unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Reject specimen if older than 16 hours post collection, refrigerated or frozen

Methodology
Lymphocyte Stimulation with Immunoassay

Setup Schedule
Collect Samples: Monday - Sunday
Testing Performed: Tuesday, Thursday, and Saturday or Sunday
BS

Report Available
4 days

Reference Range
A positive and negative control are performed with each patient sample.
If these controls are within the acceptable limits, any value <0.35 IU/ML is interpreted as negative and any value >=0.35 IU/ML is interpreted as positive. If the positive and negative control are outside the acceptable limits, the results are interpreted as indeterminate.

Clinical Significance
The QuantiFERON test is based upon a cell-mediated immune response in TB-infected individuals. The T cells of the patient are stimulated with Mycobacterium tuberculosis antigens. If the patient has previously been exposed to Mycobacterium tuberculosis, the exposure to these antigens in the QuantiFERON assay stimulate the patient's T cells to secrete the cytokine interferon-γ. The presence of the interferon-γ is then detected using an EIA method. According to the CDC, this assay may be used as a substitute for a TB skin test. It is more specific than the TB skin test, will detect latent TB, and a positive result is obtained in about 85% of the patients with active TB.

Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.