Lyme Antibody w/ Western Blot Confirmation

Message

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

Test Code

LYME

Alias/See Also

6646;
B Burgdorferi;
Borrelia B Earl;
Borrelia Burgdorferi (serum);
Borrelia Burgdorferi Antibodies;
Lyme (serum);
Lyme Disease;
Lime Disease AB Screen (Total),
EIA; Lyme Early;
Lyme IgG & IgM;&nbs

CPT Codes
86618 (reflex 86617(2))

Includes

Western blot confirmation(8593) CPT code(s) 86617x2 performed when screen is positive at an additonal charge.

Preferred Specimen

1 mL serum (minimum 0.5 mL)

Instructions

SST or Red top: Avoid hemolysis. Invert a minimum of 5 times, then allow to clot in an upright position for 30 minutes. Centrifuge the the tube for at least 10 minutes. Keep tightly stoppered.

Transport Container

SST (speckled top), Red top , Plastic Vial (transfer) tube

Transport Temperature

Refrigerated (preferred)

Specimen Stability

Room Temperature = n/a
Refrigerated = 7 days
Frozen = at least 7 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Rejected for gross hemolysis, and lipemia. Microbial contamination may interfere. Plasma will be rejected

Methodology
Enzyme Immunoassay

Setup Schedule

Tuesday and Thursday
BS

Report Available

6 days

Reference Range

SEX	AGE	Report Normals	Units
N/M/F	133Y	< OR = 0.90 Negative
		0.91 - 1.09 Equivocal
		> OR = 1.10 Positive
Due to recommendations by the food and drug administration (FDA), all positive or equivocal Borrelia Burgdorferi Antibody EIA (screening) tests will be followed by the western blot. The screening test for B. Burgdorferi has a low predicitive value for a negative result when used to detect early infection and a low predictive value of a positive result when exposure history, symptoms, and clinical findings are not consistent with lyme disease. Positive or equivocal results should not be interpreted as true positives until a second-step testing of the specimen is done using a method that is more specific for antibodies to B. Burgdorferi (e.g. Western Blot).

Clinical Significance

Lyme disease is caused by a bacterium borrelia burgdorferi and is transmitted by ticks. EIA is the screening test with high sensitivity for antibody detection. Western blot testing qualitatively examines with high specificity antibodies in a patient's specimen. Western blot testing is appropriate for confirming a detected EIA test result.

Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439

Last Updated: November 22, 2021

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.