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Bilirubin, Direct
MessageProtect specimen from light
For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.
For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.
Test Code
DBIL
Alias/See Also
285;
Bili Direct;
Conjugated Bili;
Conjugated Bilirubin;
D Bili;
Dir Bili;
Direct Bili;
Direct Bilirubin;
Bili Direct;
Conjugated Bili;
Conjugated Bilirubin;
D Bili;
Dir Bili;
Direct Bili;
Direct Bilirubin;
CPT Codes
82248
Preferred Specimen
serum
Minimum Volume
1 mL
Other Acceptable Specimens
plasma from Lithium Heparin (Light Green Top)tube
Instructions
Barrier tubes (serum separator tubes or plasma separator tubes) are preferred. If non-barrier tubes are utilized, the serum/plasma must be removed immediately after centrifuging and placed in a screw-cap transfer tube.
Collection notes: Avoid hemolysis. Invert tubes at least 8 times. Allow to clot in an upright position for 30 minutes. Within 2 hours, centrifuge tubes 10 minutes.
Keep tightly stoppered. Do NOT re-centrifuge tubes. Do NOT freeze the primary collection tube.
WRAP IN FOIL/PROTECT FROM LIGHT
Collection notes: Avoid hemolysis. Invert tubes at least 8 times. Allow to clot in an upright position for 30 minutes. Within 2 hours, centrifuge tubes 10 minutes.
Keep tightly stoppered. Do NOT re-centrifuge tubes. Do NOT freeze the primary collection tube.
WRAP IN FOIL/PROTECT FROM LIGHT
Transport Container
Original tube preferred, may be sent in a screw-top transfer tube.
Transport Temperature
Room Temperature or Refrigerated
Specimen Stability
Room Temperature = 2 days
Refrigerated = 7 days
Frozen = 6 months
Refrigerated = 7 days
Frozen = 6 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis, Lipemia, incorrect specimen type, insufficient sample volume, improper storage conditions, exceeds specimen stability guidelines, contaminated sample.
Methodology
Spectrophotometric
Setup Schedule
24/7
AU
AU
Report Available
daily
Clinical Significance
Measurement of the levels of bilirubin is used in the diagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gall bladder obstruction. The assessment of direct bilirubin is helpful in the differentiation of hepatic disorders. The increase in total bilirubin associated with obstructive jaundice is primarily due to the direct (conjugated) fraction. Both direct and indirect bilirubin are increased in the serum with hepatitis.
Performing Laboratory
Compunet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439
Last Updated: May 5, 2022
