PNH Profile (FLOW Cytometry)

Message

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

Contact Flow Cytometry (937-208-6687 or 1-800-686-2252 option 3, option 2) as soon as the specimen is collected. Any specimen collected on Friday & or the week day prior to a Holiday must be called by 4:00 pm for appropriate arrangements to be made.

Test Code

73878

Alias/See Also

Paroxysmal Nocturnal Hemoglobinuria

CPT Codes
88184(1)(first marker), 88185 x# of markers minus the first marker, 88187 or 88188 or 88189 dependent upon the number of markers interpreted.

Includes

CD45, CD2, CD3, CD4, CD5, CD7, CD8, CD19, CD20, kappa, lambda, CD14, HLA-DR, CD10, CD24, CD55, CD59, CD64, FLAER.

Preferred Specimen

VENOUS PERIPHERAL BLOOD ONLY: 5 mL peripheral blood Sodium Heparin (dark green top) tube and 5 mL peripheral blood K2EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA (lavender top) tube. (minimum 2 - 3 mLs)

Minimum Volume

VENOUS PERIPHERAL BLOOD: (minimum 2 -3 mLs)

Instructions

Sodium Heparin and K2EDTA: Avoid hemolysis. Gently invert 10 times.

Transport Container

Sodium Heparin (dark green top) tube and K2EDTA (lavender top) tube

Transport Temperature

Room Temperature and transport without delay.

Specimen Stability

VENOUS PERIPHERAL BLOOD:
     Room temperature = 24 hours post collection
      Refrigerated = unacceptable
      Frozen = unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

clotted, gross hemolysis or greater than 24 hours old; microcontainers; finger or heel sticks

Methodology
Flow Cytometry

Setup Schedule

Analysis performed Monday - Friday 7:00 am - 6:00 pm. Test not performed on weekends.
RFMVH

Report Available

1 - 2 days

Clinical Significance

To determine the presence of a PNH clone.

Performing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine, OH 45439

Last Updated: August 4, 2022

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.