Epstein-Barr Virus DNA, Qualitative Real-Time PCR

Test Code
34179

CPT Codes
87798

Preferred Specimen
1 mL whole blood or plasma collected in an EDTA (lavender-top), ACD-B (yellow-top), PPT-potassium EDTA (white-top) tube, or
1 mL serum, or
1 mL CSF submitted in a sterile plastic leak-proof container, or
3mm fresh tissue submitted in a sterile plastic leak-proof container

Minimum Volume
0.3 mL

Other Acceptable Specimens
Bronchoalveolar lavage or eye fluid collected in a sterile plastic leak-proof container

Instructions
CSF: Collect in a sterile, plastic container and store refrigerated or frozen.

Tissue: Collect in a sterile, plastic container and transport frozen.

Avoid repeated freezing and thawing of specimens. Do not freeze whole blood.

Transport Temperature
Refrigerated (cold packs)
Tissue: Frozen

Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable

All other specimens
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed whole blood • Heparin whole blood

Methodology
Real-Time Polymerase Chain Reaction

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Daily; Report available: 1 day

Reference Range
Not detected

Clinical Significance
Infection with EBV is common and is generally subclinical or presents as a self-limited illness. Reactivation of latent EBV in an immunocompromised person can lead to more serious results, including lymphoproliferative disorders or neurological disease. PCR methods may be useful in identifying EBV in a variety of clinical specimens.

Performing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly, VA 20153



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.