ANCA Panel (w/Reflex)

For Specimen Integrity during Extreme Weather see the “Lockbox Usage in Extreme Weather” document at the top of this page.

ANCAS

75847

1 mL Serum (SST or Red top)

0.5 mL

Avoid hemolysis. Invert a minimum of 5 times then allow to clot in an upright position for 30 minutes. Centrifuge the tube for at least 10 minutes. Keep tightly stoppered.

MPOPR3 added as reflex (CPT 86021)

SST (speckled top) tube

Room Temperature or Refrigerated (preferred)

Room Temperature = 1 day
Refrigerated = 8 days
Frozen = 8 days

Hemolysis, lipemia

Screen and Titer: Monday, Wednesday and Friday

7 days

Testing for Anti-Neutrophil Cytoplasmic Antibodies (P-ANCA and/or C-ANCA) has been found to be useful in establishing the diagnosis of suspected vascular diseases (WG, Crescentic Glomerulonephritis, Microscopic Polyarteritis and Churg-Strauss Syndrome). Bowel Disease (Crohn's Disease, Ulcerative Colitis, Primary Sclerosing Cholangitis, and Autoimmune Hepatitis) as well as with other autoimmune diseases (drug-induced Lupus, SLE, Felt's Syndrome).

ANCA has classically been divided into C-ANCA and P-ANCA depending on the immunofluorescent pattern observed. More recently the specific antigens responsible for these patterns have been described and isolated. The primary antigen that gives the C-ANCA pattern is Proteinase-3 (PR-3). Multiple antigens are responsible for P-ANCA pattern, the principle antigen being Myeloperoxidase (MPO). Patients with Bowel Disease have been shown to have antibodies that give a P-ANCA or C-ANCA pattern. These antibodies however may not be directed towards MPO. Patients with drug induced Lupus, etc., often present with a P-ANCA pattern that is associated with antibodies against MPO.