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COMPLEMENT, C3
MessageNOTE: Clotting process requires a total of 30-60 minutes.
- Prior to centrifugation, allow sample to clot at room temperature for 15-30 minutes in red tube.
- Refrigerate sample, allow clotting to continue for a full 30 minutes.
- Rim the clot then centrifuge in a refrigerated centrifuge. If a refrigerated centrifuge is not available, aliquot (transfer) serum and refrigerate immediately.
- If sample will not be tested within 4 hours of centrifugation, refrigerate or freeze serum in a transfer tube. Do not freeze the red tube.
Test Code
C3
CPT Codes
86160
Preferred Specimen
1 Red tube
Minimum Volume
0.3 mL
Other Acceptable Specimens
None.
Instructions
NOTE: Clotting process requires a total of 30-60 minutes.
- Prior to centrifugation, allow sample to clot at room temperature for 15-30 minutes in red tube.
- Refrigerate sample, allow clotting to continue for a full 30 minutes.
- Rim the clot then centrifuge in a refrigerated centrifuge. If a refrigerated centrifuge is not available, aliquot (transfer) serum and refrigerate immediately.
- If sample will not be tested within 4 hours of centrifugation, refrigerate or freeze serum in a transfer tube. Do not freeze the red tube.
Transport Container
Transfer tube
Transport Temperature
Refrigerate or freeze
Specimen Stability
- 2-8°C up to 8 days
- 15-30°C up to 4 hours
- Frozen up to 8 days (thaw only once)
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed
Methodology
Immunoturbidemetric
Setup Schedule
M, T, W, Th, F, Sa, Su
Report Available
Within 24 hours of receipt at the performing laboratory.
Reference Range
87 - 200 mg/dL
Clinical Significance
The Complement system is composed of a series of circulating blood proteins that serve as mediators of the inflammatory response. C3 comprises about 70% of the total protein in the complement system and is central to activation of both the classical and alternate pathways. Measurements of this protein aids in the diagnosis of immunologic disorders, especially those associated with deficiencies of complement components.
Performed By
Alverno Laboratories
Performing Laboratory
Alverno Central Lab
NCHB1 (Chemistry, Beckman AU)
Last Updated: September 24, 2024