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HLA B27
MessageThis test is no longer collected in glass ACD tubes.
Test Code
HLA27
CPT Codes
86812
Preferred Specimen
1 Lavender tube (EDTA)
Minimum Volume
0.5 mL
Other Acceptable Specimens
None
Instructions
Maintain sample at room temperature.
Transport Temperature
Room temperature
Specimen Stability
15-30°C up to 72 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Samples will be rejected if frozen
Methodology
Flow Cytometry
Reference Range
Negative, Weakly Positive, or Positive
Due to possible cross-reactivity with other HLA antigens, confirmatory testing such as microlympocytotoxicity or PCR is recommended.
Clinical Significance
Assists in the diagnosis of ankylosing spondylitis, juvenile rheumatoid arthritis and Reiter syndrome.
Performed By
Alverno Laboratories
Performing Laboratory
Alverno Central Lab
NMB10 (Molecular Biology, Flow)
Last Updated: April 25, 2023