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DIRECT ANTIGLOBULIN TEST
MessageMUST include MRN number on label
Test Code
DAT
Alias/See Also
Direct Coombs; Coombs
CPT Codes
86880
Includes
Includes: IgG & Complement (Children & Adults), IgG (Infants)
Preferred Specimen
1 Lavender tube (EDTA)
Minimum Volume
0.5 mL
Other Acceptable Specimens
1 Pink tube (EDTA)
Instructions
Do not centrifuge; May remain at room temp until testing.
Transport Temperature
Room Temperature or Refrigerate
Specimen Stability
2-8°C up to 3 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed
Methodology
Hemagglutination
Setup Schedule
M, T, W, Th, F, Sa, Sun
Reference Range
Negative
Clinical Significance
The direct antiglobulin test (DAT or direct Coombs test) is used detect antibodies attached to red blood cells. It is primarily used to help diagnose the cause of hemolytic anemia but may also be used to help diagnose hemolytic disease of the newborn (HDN) or a blood transfusion reaction.
Performed By
Alverno Laboratories
Performing Laboratory
Versiti
Last Updated: September 18, 2023