ANTIPHOSPHOLIPID SYNDROME PANEL

Test Code

ANPS1

CPT Codes
86147 x2; 86146 x2; 85610; 85730; 85520; 85670, if reflexed 85384; If results for all 4 tests above are normal, then: 85613 (up to x3); 85730 (up to x3)

Includes

Panel components: Cardiolipin antibodies IgG and IgM, Beta-2 glycoprotein antibodies IgG and IgM, PT, PTT, Thrombin Time, Anti-Xa, Fibrinogen (if indicated), dRVVT and Silica Clotting Time Screen (SCT), dRVVT and SCT confirmation (if indicated), dRVVT and SCT mixing studies.

This test includes a Pathologist interpretation.

Preferred Specimen

2 Blue tubes (Sodium Citrate)
1 Gold tube (gel-separator)

Minimum Volume

1 mL plasma
1 mL serum

Other Acceptable Specimens

None

Instructions

This test requires two sample types:

Serum: Centrifuge for complete separation of serum and red cells. Refrigerate until tested.
Plasma: Tube MUST be full for proper whole blood to anticoagulant ratio. Invert tube "end-to-end" to prevent clotting. Centrifuge to obtain platelet-poor plasma. Refer to the document Obtaining A Platelet-Poor Sample below.

Transport Temperature

Plasma: Must be frozen
Serum: Refrigerate

Specimen Stability

Plasma: Frozen plasma is stable up to 2 weeks
Serum: 2-8°C up to 7 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

The following conditions will be rejected:

Refrigerated plasma samples that are >4 hours from collection
Clotted samples
Grossly hemolyzed samples

Methodology
Clot Detection and Multiplex Flow Immunoassay

Setup Schedule

T, Th only

Report Available

Within 24 hours of the day tested.

Reference Range

Component	Reference Range
Cardiolipin Antibody, IgG	<20 GPL
Cardiolipin Antibody, IgM	<20 GPL
Beta-2 Glycoprotein, IgG	<20 U/mL
Beta-2 Glycoprotein, IgM	<20 U/mL
PT	10.1 - 13.1 sec
PTT	25.0 - 36.0 sec
Thrombin Time	10.3 - 16.6 sec
Anti-Xa	Not Present
Fibrinogen (if indicated)	163 - 463 mg/dL
dRVVT Screen Ratio	<1.20
Silica Clotting Time Screen Ratio (SCT)	<1.22
dRVVT Confirmation Total Ratio (if indicated)	<1.20
Silica Clotting Time Confirmation (SCT) Total Ratio (if indicated)	<1.22
dRVVT Screen Mixing Study Ratio (if indicated)	<1.20
Silica Clotting Time Confirmation (SCT) Screen Mixing Study Ratio (if indicated)	<1.22

Clinical Significance

Preferred initial panel when antiphospholipid syndrome (APS) is highly suspected.

Performed By
Alverno Laboratories

Performing Laboratory
Alverno Central Lab
NHMC (Hematology, IL Coag)

Additional Information
Obtaining A Platelet-Poor Sample
BD Vacutainer Plus Blue Top Volume Fill Guide

Last Updated: July 6, 2023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.