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REQUEST FISH (Process and Hold Only)
MessageSample MUST be submitted with a Hematopathology Patient Information Sheet.
Test Code
RQFSH
Alias/See Also
FISH fix and hold
Preferred Specimen
Bone Marrow or Whole Blood: 1 Green Tube (Sodium Heparin)
Lymph Node or Biopsy: RPMI
Lymph Node or Biopsy: RPMI
Minimum Volume
- Bone marrow 1 mL
- Whole blood 1 mL
- Tissue or lymph node 5x5 mm
Other Acceptable Specimens
Bone Marrow or Whole Blood: 1 Lavender tube EDTA
Transport Temperature
Whole blood or bone marrow: Room temperature
Lymph node or tissue: Refrigerate in RPMI
Lymph node or tissue: Refrigerate in RPMI
Specimen Stability
- Whole blood or bone marrow: 15-30°C up to 2 days
- Lymph node or tissue: 2-8°C in RPMI up to 2 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Clotted. Irretrievable samples will not be rejected.
Clinical Significance
Samples for potential FISH testing will need this code ordered. This code will allow the sample to be processed, fixed, and stored for up to 2 weeks. The RBC’s will be lysed and WBC’s will be fixed and frozen. This process cannot be used for add on Multiple Myeloma Panel by FISH (MMYFH)
Any add on FISH testing must be requested within 2 weeks sample fixing; refer to specific FISH Panel test code(s)
The Pathologist must call the Alverno Central Laboratory Client Services department to add-on a specific Heme FISH panel.
Automatic reflex FISH testing will not be performed
Any add on FISH testing must be requested within 2 weeks sample fixing; refer to specific FISH Panel test code(s)
The Pathologist must call the Alverno Central Laboratory Client Services department to add-on a specific Heme FISH panel.
Automatic reflex FISH testing will not be performed
Performed By
Alverno Laboratories
Additional Information
Hematopathology Patient Information Sheet
Last Updated: April 17, 2024
