Resources

CHRONIC LYMPHOPROLIFERATIVE PANEL BY FLOW CYTOMETRY

Message
Physician must be contacted for approval before Add-On testing can be submitted


MUST include Hematopathology Patient Information Sheet with order.  Form is linked in the Additional Information below, in the Resources section, on our website, or by calling Client Services at 219-989-3700.




Test Code
FLWCY


Alias/See Also
CLL


CPT Codes
88184;88185 x17;88189

Includes

Includes: CD45 X SSC Gating: CD8; CD4; CD3; CD5; CD7; CD6; CD2; CD38; CD56; CD20; CD19; CD10; CD11c; Kappa; Lambda; CD38; FMC7; CD23




Preferred Specimen
Whole blood or bone marrow:  collect 1 Lavender tube (EDTA)

Lymph node tissue or any tissue source:  Place tissue sample in sterile container containing RPMI Media

Please note that tissue submitted in sterile saline is stable for 4 hours only



Minimum Volume
Sample Type Minimum Volume
Whole blood 2 mL
Bone marrow 1 mL
Body fluid 10 mL
Tissue/Lymph node 5x5 mm


Other Acceptable Specimens
1 Green tube (Sodium Heparin) Sodium Heparin will be accepted for peripheral blood or bone marrow - do not send Lithium Heparin tube


Instructions
Deliver specimen to laboratory ASAP.  Must include Hematopathology Patient Information Sheet with order.  Form is linked in the Additional Information below, in the Resources section, on our website, or by calling Client Services at 219-989-3700.


Transport Temperature
  • Blood or bone marrow: store at room temperature up to 48 hours
  • Fresh tissue or lymph node: store at 2-8ºC in RPMI media up to 48 hours
  • Body fluid: store at room temperature up to 24 hours


Specimen Stability
  • Blood or bone marrow: store at room temperature up to 48 hours
  • Fresh tissue or lymph node: store at 2-8ºC in RPMI media up to 48 hours
  • Body fluid: store at room temperature up to 24 hours


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimen too old for analysis or improperly stored.
Irretrievable samples will not be rejected.


Methodology

Flow Cytometry



Setup Schedule

M, T, W, Th, F, Sa
Note:  Collections must be received at the Central Lab no later than 11:00 am on Saturday.




Report Available
Report available within 48 business hours from receipt.


Reference Range

See interpretative pathology report (SoftPath)




Clinical Significance

Fresh tissue, lymph nodes, peripheral blood and bone marrow for lymphoma studies (ex. CLL, MM, NHL)




Performed By
Alverno Laboratories

Performing Laboratory
Alverno Central Lab  
NMB10 (Molecular Biology, Flow)

Additional Information
Hematopathology Patient Information Sheet

Last Updated: April 17, 2024


The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.