KRAS MUTATION by PCR

Message

This assay detects mutation s in codons 12, 13, 59, 61, 117 and 146.

Test Code

KRAMT

CPT Codes
81275; 81276; 88381

Includes

**Mutation Detections**
Codon	Mutation
12	p.Gly12Ala; p.Gly12Cys; p.Gly12Asp; p.Gly12Arg; p.Gly12Ser; p.Gly12Val
13	p.Gly12Asp
59	p.Ala59Thr/Glu/Gly
61	p.Gin61His; p.Gin61Lys; p.Gin61Leu/Arg
117	p.Lys117Asn
146	p.Ala146Pro/Thr

Preferred Specimen

Please send 2-5 slides (≥50mm tissue area) or paraffin block and 1 H&E slide with the area of invasive tumor circled. Send tissue block to the Molecular department in a red pathology interoffice envelope.

Transport Temperature

Room temperature

Specimen Stability

Room temperature. Cut slides are stable 60 days. Paraffin blcoks are stored for 10 years.

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Less than 10% tumor cells
Decalcified specimens
Samples sent frozen

Methodology
Real-time Polymerase Chain Reaction (PCR)

Setup Schedule

Batched; 2-3 times per week

Report Available

Report available within 1-3 days of receipt at the performing laboratory.

Reference Range

KRAS Mutation Result: Not Detected
KRAS Status: Wildtype

Clinical Significance

Aids in treatment decisions in individuals with solid tumors including colorectal and non-small cell lung cancers.

Performed By
Alverno Laboratories

Performing Laboratory
Alverno Central Lab
NMB18 (Molecular Biology, Idylla)

Last Updated: June 8, 2023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.