IMMUNOTYPING, URINE, 24 HOUR or RANDOM

Message

Aliquot (transfer) urine into one (1) FULL 10 mL urine tube and one (1) 6mL no additive vacutainer tube (Urine Chemistry tube) and note the total volume.

Test Code

IEP-F

Alias/See Also

Immunofixation; IFE; Urine protein immunoelectrophoresis; Electrophoresis; IEPF

CPT Codes
86335

Includes

Monoclonal paraprotein identification (if present) along with Pathologist Interpretation.

Preferred Specimen

24 Hour Urine Container without preservative (aliquot or transfer urine into a transfer tube and note the total volume)

Minimum Volume

15 mL aliquot

Other Acceptable Specimens

Sterile Container for random urine

Instructions

Mix the specimen well and measure the 24-Hour volume
RECORD THE TOTAL VOLUME
Aliquot (transfer) urine into one (1) FULL 10 mL urine tube and one (1) 6mL no additive vacutainer tube (Urine Chemistry tube); note the total volume.
Refrigerate if not tested immediately

Transport Temperature

Refrigerate

Specimen Stability

2-8°C up to 7 days (samples may be stored separately in Gen Lab)

Methodology
Capillary electrophoresis

Setup Schedule

M, T, W, Th, F

Report Available

Report available within 24 hours M-F. Samples collected on Friday will be reported on Monday.

Reference Range

No monoclonal paraproteins observed

Clinical Significance

Ideally, this test is performed after the protein electrophoresis has identified the presence of a monoclonal paraprotein. If the patient has a known or previous M-spike, both the immunotyping and serum electrophoresis are ordered at the same time.

Urine IFE is used for the detection of monoclonal proteins as seen in monclonal gammopathies (MGUS, MM, smoldering myeloma) and amyloidosis.

Performed By
Alverno Laboratories

Performing Laboratory
Alverno Central Lab
NCH4 (Chemistry, Sebia Electrophoresis)

Additional Information
24 Hour Urine Collection

Last Updated: January 23, 2024

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.