CD4 & CD8 COUNT

Message

CD8 is not orderable as a single-order test.

Test Code

CD48C

CPT Codes
86359; 86360

Includes

CD48C test includes:
Absolute Lymphocytes, Percentage CD3, Absolute CD3, Percentage CD4, Absolute CD4, Percentage CD8, Absolute CD8, CD4/CD8 Ratio (calculated)

Preferred Specimen

1 Lavender tube (EDTA) Primary tube must be submitted - cannot perform this test as an add-on.

Minimum Volume

2 mL

Instructions

Deliver specimen to laboratory ASAP. Submit whole blood, do not centrifute.

Transport Temperature

Room temperature

Specimen Stability

Room temperataure up to 48 hours
(Test set up as batch, early am only)

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Specimen too old for analysis or improperly stored. Plasma samples will not be accepted.

Methodology
Flow cytometry

Setup Schedule

M, T, W, Th, F, Sa

Report Available

Report available within 24 hours of receipt Monday - Saturday

Report available within 48 hours of receipt Sunday and holidays

Reference Range

CD3%	58-84
CD3 cells/uL	856-2237
CD4%	34-65
CD4 cells/uL	518-1472
CD8%	13-38
CD8 cells/uL	205-924
CD4/CD8 Ratio	0.9-5.0

Clinical Significance

Serial monitoring of CD4 T-cell count in HIV-positive patients. Useful for follow-up and diagnostic evaluation of primary cellular immunodeficiencies including severe combined immunodeficiency.

Performed By
Alverno Laboratories

Performing Laboratory
Alverno Central Lab
NMB10 (Molecular Biology, Flow)

Last Updated: April 8, 2024

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.