Centrifuge within 2 hours of collection. If testing cannot be completed within 48 hours, freeze. Each sample MUST be labeled with the time of draw.

This test consists of three (3) phlebotomy draws: Baseline (pre-injection); 30 min (post-injection); and a 60 min (post-injection)

Test Code

CPT Codes
80400; 82533


Baseline cortisol (pre-injection); 30 min cortisol (post-injection); 60 min cortisol (post-injection)

Preferred Specimen
1 Gold tube (gel separator)

Minimum Volume

0.2 mL

Other Acceptable Specimens
Green tube (Heparin)
Lavender tube (EDTA)


Allow specimen to clot completely at room temperature. Separate serum or plasma from cells ASAP or within 2 hours of collection. Specimen must be labeled with time drawn.

Transport Temperature


Specimen Stability

2-8°C up to 2 days; 15-30°C up to 8 hours; Frozen samples are stable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolyzed samples will be rejected.


Setup Schedule

M, T, W, Th, F, Sa, Sun

Reference Range
A normal response is defined as a cortisol level of >18-20 ug/dL at any time point OR an increment of >7.3 ug/dL from the baseline cortisol level.

Clinical Significance

Used as an aid in the diagnosis and treatment of disorders of the adrenal gland

Performed By
Alverno Laboratories

Performing Laboratory
Alverno Central Lab  
NCHB2 (Chemistry, Beckman DxI)

Last Updated: April 25, 2023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.