25-HYDROXYVITAMIN D2 AND D3

Message

This test is an alternative screening test for Vitamin D deficiency and monitoring response in patients that are not responding to supplementation.

Test Code

VTD25

CPT Codes
82306

Preferred Specimen

1 Gold tube (gel separator)

Minimum Volume

0.5 mL

Other Acceptable Specimens

or 1 Green tube (Lithium Heparin)

Instructions

Allow serum samples to clot at room temperature. Centrifuge for complete separation of serum.

Transport Temperature

Refrigerate

Specimen Stability

2-8ºC up to 7 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis, lipemia, or gross icterus

Methodology

LC/MS/MS

Setup Schedule

Monday, Wednesday, and Friday; day shift only

Reference Range

Vitamin D 25-OH, Total

30-100 ng/mL

Values for Vitamin D 25-OH D3 and D2 not established

Clinical Significance

Vitamin D non-response to therapy (25-OH Vit D2, D3) is used to evaluate the non-response to supplemental therapy. Only order after 25-Hydroxy Vitamin D levels have been tested.

Performed By
Alverno Laboratories

Performing Laboratory
Alverno Central Lab
NCHMS (LC/MS/MS, Mass Spec)

Last Updated: April 25, 2023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.