Biotinidase

Test Code

BITIN

Alias/See Also

70132

CPT Codes
82261

Preferred Specimen

2 mL serum collected in a serum separator tube (SST)

Minimum Volume

1 mL

Other Acceptable Specimens

Plasma collected in an EDTA (lavender-top) tube, or sodium or lithium heparin (green-top) tube

Instructions

Separate serum or plasma within one hour of collection and store at minimum -20° C or below. Use dry ice for shipment.

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis

Methodology
Colorimetric (C) • Enzymatic

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Tuesday, Thursday Morning Report available: 3 Days after receipt at the performing laboratory. Add three (3) days for transport.

Reference Range

5.1-11.9 nmol/mL/min

Clinical Significance

Detect biotinidase deficiency. Children born with biotinidase deficiency develop mental retardation; it is a very treatable disorder once diagnosed.

Performing Laboratory

Quest Diagnostics Nichols Institute-San Juan Capistrano, CA

33608 Ortega Highway

San Juan Capistrano, CA 92675-2042

Last Updated: May 4, 2023

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.