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Cytomegalovirus (CMV) DNA, Qualitative Real-Time PCR : 10601
Test CodeCMVQL or 10601
CPT Codes
87496
Instructions
Whole blood: Collect whole blood in sterile tubes containing EDTA as anticoagulant. Store refrigerated. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw capped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum, CSF, Amniotic Fluid, Urine, Eye fluid and Bronchoalveolar Lavage: Collect in a sterile container and store refrigerated or frozen.
Fresh (unfixed) tissue: Collect in a sterile container and store frozen.
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tubes (PPTs). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw capped tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum, CSF, Amniotic Fluid, Urine, Eye fluid and Bronchoalveolar Lavage: Collect in a sterile container and store refrigerated or frozen.
Fresh (unfixed) tissue: Collect in a sterile container and store frozen.
Transport Container
Preferred Specimens
1 mL (Minimum 0.5 mL) Whole blood collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Plasma collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Serum Collect in a red‐top tube (no gel) or Serum Separator Tube(SST®).
1 mL (Minimum 0.5 mL) CSF, bronchoalveolar lavage, eye fluids, amniotic fluid amniotic fluid supernatant, random urine collected in a sterile leak‐proof container.
Alternative Specimen
3 mm frozen fresh tissue.
1 mL (Minimum 0.5 mL) Whole blood collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Plasma collected in an EDTA (lavender‐top) tube.
1 mL (Minimum 0.5 mL) Serum Collect in a red‐top tube (no gel) or Serum Separator Tube(SST®).
1 mL (Minimum 0.5 mL) CSF, bronchoalveolar lavage, eye fluids, amniotic fluid amniotic fluid supernatant, random urine collected in a sterile leak‐proof container.
Alternative Specimen
3 mm frozen fresh tissue.
Transport Temperature
Refrigerated.
Fresh tissue and stored frozen specimens: Transport Frozen
Fresh tissue and stored frozen specimens: Transport Frozen
Specimen Stability
Whole blood
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
All other sample types
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
All other sample types
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Sunday - Saturday
Report Available
1-3 days
Reference Range
Not Detected
Clinical Significance
In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV is also the most common cause of congenital viral infection in humans. Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients or in determining the viral load of CMV in amniotic fluid.
Performing Laboratory
med fusion
