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Cytomegalovirus (CMV) DNA, Qualitative Real-Time PCR : 10601
Test CodeCMVQL or 10601
CPT Codes
87496
Preferred Specimen
1 mL (0.5 mL minimum) whole blood collected in an EDTA (lavender-top) tube or
Plasma collected in an EDTA (lavender-top) or
PPT potassium EDTA tube or
Serum or
CSF, amniotic fluid, bronchoalveolar lavage (BAL) or random urine collected in a sterile, plastic, leak-proof container or
0.3 mL (0.25 mL minimum) undiluted vitreous (eye) fluid collected in a sterile, plastic, leak-proof container
Plasma collected in an EDTA (lavender-top) or
PPT potassium EDTA tube or
Serum or
CSF, amniotic fluid, bronchoalveolar lavage (BAL) or random urine collected in a sterile, plastic, leak-proof container or
0.3 mL (0.25 mL minimum) undiluted vitreous (eye) fluid collected in a sterile, plastic, leak-proof container
Other Acceptable Specimens
3 mm frozen tissue
Instructions
Whole blood: Collect whole blood in sterile tubes containing EDTA anticoagulant. Store refrigerated. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA anticoagulant or in plasma preparation tubes (PPT). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-cap tubes and store refrigerated or frozen. If blood is collected in a PPT, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT to aliquot tubes.
Serum: Collect in a red-top tube (no gel) or serum separator tube (SST) and store refrigerated or frozen.
CSF, amniotic fluid, urine, BAL: Collect in a sterile, plastic, leak-proof container and store refrigerated or frozen.
Raw vitreous (eye) fluid specimens: Do not dilute collected specimen with additional saline or other transport media
Tissue: Fresh (unfixed) tissue submitted in a sterile, plastic, leak-proof container and store refrigerated or frozen.
Plasma: Collect blood in sterile tubes containing EDTA anticoagulant or in plasma preparation tubes (PPT). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-cap tubes and store refrigerated or frozen. If blood is collected in a PPT, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT to aliquot tubes.
Serum: Collect in a red-top tube (no gel) or serum separator tube (SST) and store refrigerated or frozen.
CSF, amniotic fluid, urine, BAL: Collect in a sterile, plastic, leak-proof container and store refrigerated or frozen.
Raw vitreous (eye) fluid specimens: Do not dilute collected specimen with additional saline or other transport media
Tissue: Fresh (unfixed) tissue submitted in a sterile, plastic, leak-proof container and store refrigerated or frozen.
Transport Temperature
Refrigerated.
Fresh tissue and stored frozen specimens: Transport Frozen
Fresh tissue and stored frozen specimens: Transport Frozen
Specimen Stability
Whole blood
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
All other sample types
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
All other sample types
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Sunday - Saturday
Report Available
1-3 days
Limitations
This test was developed and its analytical performance
characteristics have been determined by Quest Diagnostics.
It has not been cleared or approved by the U.S. Food and
Drug Administration. This assay has been validated pursuant
to the CLIA regulations and is used for clinical purposes.
characteristics have been determined by Quest Diagnostics.
It has not been cleared or approved by the U.S. Food and
Drug Administration. This assay has been validated pursuant
to the CLIA regulations and is used for clinical purposes.
Reference Range
Not Detected
Clinical Significance
In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV is also the most common cause of congenital viral infection in humans. Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients or in determining the viral load of CMV in amniotic fluid.
Performing Laboratory
med fusion
