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BK Virus DNA, Quantitative, Real-Time PCR, Plasma : 11274
MessageFor CSF order test code 18901 BKVCSF, for Urine order test code 16581 BKUQNT
Test Code
BKPQNT or 11274
CPT Codes
87799
Instructions
Plasma: Collect blood in sterile tubes containing EDTA as an anticoagulant, or in plasma preparation tube (PPT). Collect whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, transport aliquot tubes. Freeze and ship frozen.
Serum: Collect blood in sterile tubes with no anticoagulants; serum separator tubes (SST) are recommended. Allow blood to clot at room temperature and separate serum within 1 hour of collection. Transfer serum to sterile, transport aliquot tubes. Freeze and ship frozen.
Whole blood: Collect using standard procedures. Store samples refrigerated following collection. Do not freeze whole blood.
Serum: Collect blood in sterile tubes with no anticoagulants; serum separator tubes (SST) are recommended. Allow blood to clot at room temperature and separate serum within 1 hour of collection. Transfer serum to sterile, transport aliquot tubes. Freeze and ship frozen.
Whole blood: Collect using standard procedures. Store samples refrigerated following collection. Do not freeze whole blood.
Transport Container
Preferred Specimens
.7 mL plasma collected in an EDTA (lavender-top) tube.
or
0.7 mL serum collected in a serum separator tube (SST) .
or
0.7 mL whole blood collected in an EDTA (lavender-top) tube).
Minimum Volume
0.3 mL
.7 mL plasma collected in an EDTA (lavender-top) tube.
or
0.7 mL serum collected in a serum separator tube (SST) .
or
0.7 mL whole blood collected in an EDTA (lavender-top) tube).
Minimum Volume
0.3 mL
Transport Temperature
Plasma or serum : Frozen
Whole blood : Refrigerated
Whole blood : Refrigerated
Specimen Stability
Plasma and serum
Room temperature: 48 hours; Refrigerated: 7 days; Frozen: 30 days
Whole blood
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
Room temperature: 48 hours; Refrigerated: 7 days; Frozen: 30 days
Whole blood
Room temperature: 48 hours; Refrigerated: 8 days; Frozen: Unacceptable
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Sunday - Saturday
Report Available
1-3 days
Reference Range
Not Detected
Clinical Significance
This test is used to determine the presence of BK Virus DNA in patient's specimens. Detection of the virus in these specimens may be indicative of an active infection, as PCR detects the presence of the virus, and not the host's reaction to the virus. Polyomavirus BK DNA detection in urine or plasma is associated with an increased risk of graft rejection in renal recipients. Quantitative testing may indicate change in risk over time. This is a quantitative molecular test, with a linear range of 500-500,000,000 copies/mL ( 2.70-8.70 Log copies/mL)
Performing Laboratory
med fusion
