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Antithrombin III Activity : 216
Test CodeAT3ACT or 216
CPT Codes
85300
Instructions
Submit a separate frozen vial for each special coagulation assay ordered. Draw blood in a light blue-top tube containing 3.2% sodium citrate. Mix gently by inversion 3-4 times. Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice.
Transport Container
Preferred Specimen
1 mL frozen platelet-poor plasma collected in 3.2% Sodium citrate (light blue-top) tube
Minimum Volume
0.5 mL
1 mL frozen platelet-poor plasma collected in 3.2% Sodium citrate (light blue-top) tube
Minimum Volume
0.5 mL
Transport Temperature
Frozen.
Specimen Stability
Room temperature: Unacceptable; Refrigerated: Unacceptable; Frozen: 30 days
Methodology
Chromogenic Substrate
Setup Schedule
Monday - Friday
Report Available
1-5 days
Reference Range
80-135 % normal (≥ 6 months)
Clinical Significance
Aids in the detection of hypercoagulable states associated with venous thrombotic episodes. May be useful in patients who appear to be hyporesponsive to heparin.
Performing Laboratory
med fusion