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Vitamin B1 (Thiamine), Plasma/Serum, LC/MS/MS : 90353
Test CodeVB1TPS or 90353
Alias/See Also
Thiamin, B1
CPT Codes
84425
Instructions
Plasma: Collect blood in a lavender-top tube. Remove plasma within 4 hours of collection and transfer to a light protected pour off tube. If separation of cells can't be performed immediately after collection, keep the whole blood refrigerated and protected from light. The separation of cells must be completed within 4 hours of collection. Separate cells by centrifugation at 2-8° C for 8-10 minutes. Transfer plasma to amber tube to protect from light and freeze tube immediately. Alternately, neutral colored tubes can be used if wrapped in aluminum foil. Ship frozen tubes at -10 to -30° C.
Serum: Collect blood in plain red top evacuated tube. Allow to clot at 20-25° C for 20-30 minutes. Centrifuge at 2-8° C for 8-10 minutes. Transfer serum to an amber polypropylene or polyethylene transport tube to protect from light and freeze tube immediately. Alternately, neutral colored polypropylene or polyethylene tubes can be used if wrapped in aluminum foil.
Serum: Collect blood in plain red top evacuated tube. Allow to clot at 20-25° C for 20-30 minutes. Centrifuge at 2-8° C for 8-10 minutes. Transfer serum to an amber polypropylene or polyethylene transport tube to protect from light and freeze tube immediately. Alternately, neutral colored polypropylene or polyethylene tubes can be used if wrapped in aluminum foil.
Transport Container
Overnight fasting is required.
Preferred Specimen
2 mL frozen plasma collected in an EDTA (lavender-top) tube - protected from light
Alternative Specimens
Serum from plain red top tube in Amber transport tube or foil wrapped transport tube protected from light
Minimum Volume
1 mL
Preferred Specimen
2 mL frozen plasma collected in an EDTA (lavender-top) tube - protected from light
Alternative Specimens
Serum from plain red top tube in Amber transport tube or foil wrapped transport tube protected from light
Minimum Volume
1 mL
Transport Temperature
Frozen.
Specimen Stability
Room temperature: 8 hours; Refrigerated: 48 hours; Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis, Lipemia, Received room temperature, Received refrigerated, Received not protected from light
Methodology
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)
Setup Schedule
Tuesday -Saturday
Report Available
2-3 days
Reference Range
Adult: 8-30 nmol/L
Clinical Significance
Vitamin B1 deficiency is most often associated with alcoholism, chronic illness and following gastric by-pass surgery. Prolonged deficiency causes beriberi. Plasma vitamin B1 is useful in evaluating nutritional assessment and compliance, while whole blood vitamin B1 is useful in evaluating body stores.
Performing Laboratory
med fusion
