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Lymphocyte Subset Panel 5 : 8360
MessagePlease submit a copy of the most recent CBC with differential report and any clinical information related to the patient's disease state. The CBC w/diff is needed for the reporting of the flow cytometry interpretative results.
Test Code
CD4PF or 8360
Alias/See Also
CD4; Immunophenotyping (CD4)
CPT Codes
86361
Includes
Panel includes: CD4 and Absolute Lymphocytes.
Instructions
Peripheral blood in a lavender top (EDTA) tube (4-10 mL).
Transport Container
The specimen must be received within 36 hours of collection. Do not centrifuge.
Transport Temperature
Room temperature within 36 hours.
Specimen Stability
Lavender (EDTA): Room temperature: 36 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
<85% Viability for Lymphocytic Subset panels; Centrifuged specimen.
Methodology
Immunophenotyping by Flow Cytometry
Setup Schedule
Sunday - Saturday
Report Available
1-2 days
Limitations
Laboratory test results should always be considered in the context of clinical observations. This test was developed and its performance characteristics determined by med fusion. It has not been cleared or approved by the U.S. Food and Drug Administration (FDA). The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA) as qualified to perform high complexity clinical laboratory testing.
Reference Range
Age CD4 Absolute CD4 Percentage (%)
0-3 Months 1600-4000 35-64
3-6 Months 1800-4000 35-56
6-12 Months 1400-4300 31-56
1-2 Years 1300-3400 32-51
2-6 Years 700-2200 16-30
6-12 Years 650-1500 31-47
12-18 years 530-1300 31-52
Adult 490-1740 30-61
Absolute Lymphocytes 850-3900 cells/uL
0-3 Months 1600-4000 35-64
3-6 Months 1800-4000 35-56
6-12 Months 1400-4300 31-56
1-2 Years 1300-3400 32-51
2-6 Years 700-2200 16-30
6-12 Years 650-1500 31-47
12-18 years 530-1300 31-52
Adult 490-1740 30-61
Absolute Lymphocytes 850-3900 cells/uL
Clinical Significance
The measurement of lymphocyte subsets may be useful in monitoring immunosuppressive therapies and determining the immune status of patients. These quantitative tests are measured out in absolute numbers which are determined from different cell types such as total T-cells, CD4 T-cells, CD8 T-cells, B-cells, and NK cells. For example, CD4 counts are commonly used for monitoring HIV infected patients
Performing Laboratory
med fusion
