Lymphocyte Subset Panel 1 : 7197

Message

Please submit a copy of the most recent CBC with differential report and any clinical information related to the patient's disease state. The CBC w/diff is needed for the reporting of the flow cytometry interpretative results.

Test Code

LYSUBF or 7197

Alias/See Also

Immunophenotyping (Lymphocyte subset); CD4 (Lymphocyte subset); CD3 (Lymphocyte subset); CD8 (Lymphocyte subset); CD19 (Lymphocyte subset); CD56 (Lymphocyte subset); CD4/CD8 ratio (Lymphocyte subset)

CPT Codes
86359, 86360, 86355, 86357

Includes

Panel includes: CD3, CD4, CD8, CD19, NK CELLS CD56+CD16, CD4/CD8 ratio, and Absolute Lymphocytes.

Instructions

Peripheral blood in a lavender top (EDTA) tube (4-10 mL).

Transport Container

The specimen must be received within 36 hours of collection. Do not centrifuge.

Transport Temperature

Room temperature within 36 hours.

Specimen Stability

Lavender (EDTA): Room temperature: 36 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

<85% Viability for Lymphocytic Subset panels; Centrifuged specimens.

Methodology
Immunophenotyping by Flow Cytometry

Setup Schedule

Sunday - Saturday

Report Available

1-2 days

Limitations

Laboratory test results should always be considered in the context of clinical observations. This test was developed and its performance characteristics determined by med fusion. It has not been cleared or approved by the U.S. Food and Drug Administration (FDA). The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA) as qualified to perform high complexity clinical laboratory testing.

Reference Range

Age C3 Absolute CD3 Percentage (%)
0-3 Months 2500-5500 53- 84
3-6 Months 2500-5600 51-77
6-12 Months 1900-5900 49-76
1-2 Years 2100-6200 53-75
2-6 Years 1400-3700 56-75
6-12 Years 1200-2600 60-76
12-18 years 1000-2200 56-84
Adult 840-3060 57-85

Age CD4 Absolute CD4 Percentage (%)
0-3 Months 1600-4000 35-64
3-6 Months 1800-4000 35-56
6-12 Months 1400-4300 31-56
1-2 Years 1300-3400 32-51
2-6 Years 700-2200 16-30
6-12 Years 650-1500 31-47
12-18 years 530-1300 31-52
Adult 490-1740 30-61

Age CD8 Absolute CD8 Percentage (%)
0-3 Months 560-1700 12-28
3-6 Months 590-1600 12-23
6-12 Months 500-1700 12-24
1-2 Years 620-2000 14-30
2-6 Years 490-1300 16-30
6-12 Years 370-1100 18-35
12-18 years 330-920 18-35
Adult 180-1170 12-42

Age CD4:8 Ratio
0-3 Months 2.1-12.5
3-6 Months 2.1-12.5
6-12 Months 0.6-5.3
1-2 Years 0.6-5.3
2-6 Years 1.4-3.4
6-12 Years 1.0-3.3
12-18 years 0.6-3.9
Adult 0.86-5.00

Age CD19 Absolute CD19 Percentage (%)
0-3 Months 300-2000 6-32
3-6 Months 430-3000 11-41
6-12 Months 610-2600 14-37
1-2 Years 720-2600 16-35
2-6 Years 390-1400 14-33
6-12 Years 270-860 13-27
12-18 years 110-570 6-23
Adult 110-660 6-29

Age CD56 Absolute CD56 Percentage (%)
0-3 Months 170-1100 4-18
3-6 Months 170-830 3-14
6-12 Months 160-950 3-15
1-2 Years 180-920 3-15
2-6 Years 130-720 4-17
6-12 Years 100-480 4-17
12-18 years 70-480 3-22
Adult 70-760 4-25

Absolute Lymphocytes 850-3900 cells/uL

Clinical Significance

The measurement of lymphocyte subsets may be useful in monitoring immunosuppressive therapies and determining the immune status of patients. These quantitative tests are measured out in absolute numbers which are determined from different cell types such as total T-cells, CD4 T-cells, CD8 T-cells, B-cells, and NK cells. For example, CD4 counts are commonly used for monitoring HIV infected patients

Performing Laboratory
med fusion

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.