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Glucose Tolerance Test, 6 specimens : 10562
Test CodeGPC3AU or 10562
CPT Codes
82951, 82952x3
Instructions
Patient Preparation:
Fasting required. Fasting is defined as no consumption of food or beverage other than water for at least 8 hours before testing.
Immediately after a fasting specimen is obtained, have patient ingest a 75 g load of glucose. Draw subsequent specimens 1, 2, 3, 4 and 5 hours later. Label each with the specific draw time.
Serum and plasma submissions must be separated from cells.
Fasting required. Fasting is defined as no consumption of food or beverage other than water for at least 8 hours before testing.
Immediately after a fasting specimen is obtained, have patient ingest a 75 g load of glucose. Draw subsequent specimens 1, 2, 3, 4 and 5 hours later. Label each with the specific draw time.
Serum and plasma submissions must be separated from cells.
Transport Container
Preferred Specimen
Six - 1 mL plasma specimens collected in fluoride/oxalate (gray-top) tubes
Alternative Specimens
Plasma from fluoride/oxalate (gray-top) tube in a plastic screw-cap vial, Serum (separated from cells immediately) in a plastic screw-cap vial, Spun SST, Plasma from lithium heparin (green-top) tube in a plastic screw-cap vial, Plasma from sodium heparin (green-top) tube in a plastic screw-cap vial
Minimum Volume
0.5 mL for each specimen
Six - 1 mL plasma specimens collected in fluoride/oxalate (gray-top) tubes
Alternative Specimens
Plasma from fluoride/oxalate (gray-top) tube in a plastic screw-cap vial, Serum (separated from cells immediately) in a plastic screw-cap vial, Spun SST, Plasma from lithium heparin (green-top) tube in a plastic screw-cap vial, Plasma from sodium heparin (green-top) tube in a plastic screw-cap vial
Minimum Volume
0.5 mL for each specimen
Transport Temperature
Room temperature.
Specimen Stability
Serum:
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days
Whole blood (gray-top) tube:
Room temperature: 24 hours
Refrigerated: 72 hours
Frozen: Unacceptable
Sodium fluoride plasma:
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Heparinized plasma:
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 28 days
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days
Whole blood (gray-top) tube:
Room temperature: 24 hours
Refrigerated: 72 hours
Frozen: Unacceptable
Sodium fluoride plasma:
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Heparinized plasma:
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unspun SST, Red-top or non-plasma separator (PST) green-top tubes, Anticoagulants other than lithium heparin, sodium heparin or fluoride/oxalate
Methodology
Spectrophotometry
Setup Schedule
Sunday - Saturday
Report Available
1 day
Reference Range
Glucose Value (mg/dL)
Interpretation Fasting 1 Hr Tolerance 2 Hr Tolerance
Normal <100 Not established <140
Impaired Fasting 100-125
Impaired Tolerance 140-199
Diabetes ≥126* ≥200*
*Must be confirmed by testing on a subsequent day.
Clinical Significance
This test is used for the routine diagnosis of diabetes in children and the non- pregnant adult. For pregnant females use test code 6745. For appropriate interpretation of this test, the patient must fast overnight and ingest a 75 g load of glucose, immediately after a fasting specimen is obtained. (for children, the glucose load is 1.75 g/kg of body weight, up to 75). The diagnosis of diabetes is made if glucose exceeds 200 mg/dL in the 2 hour specimen and in any preceding specimen.
Performing Laboratory
med fusion