FLT3 ITD and TKD Mutation Detection by PCR and Fragment Analysis : 1017058

Test Code

MDFCPFLT3 or 1017058

Alias/See Also

TKD FLT3; ITD FLT3

CPT Codes
81245, 81246

Transport Container

Submit 3-5 mL Blood or 2-3 mL Bone Marrow in a lavender EDTA top or Sodium heparin tube. Paraffin embedded tissue block or unstained slides (5-10) at a minimum 5 micron thickness also acceptable.

Transport Temperature

Blood and Bone Marrow: Refrigerated; Tissue: Room temperature or Ice Pack in summer.

Specimen Stability

Blood and Bone marrow: 15 days Refrigerated
Tissue: Indefinitely Room temperature or Refrigerated

Methodology
PCR and Fragment Analysis

Setup Schedule

Monday & Wednesday & Friday

Report Available

3-5 days

Reference Range

An interpretive report will be provided.

Clinical Significance

FLT3 mutations represent some of the most frequent molecular changes in acute myeloid leukemia (AML) and are reported in 25% to 30% of AML patients. These mutations include FLT3 ITDs in the region encoding the juxtamembrane region and point mutations in the TKD; these mutations promote malignancy by constitutively activating FLT3 kinase activity without the need for the ligand. FLT3 ITD mutations with a high mutant allelic ratio (≥0.5) are associated with a poor prognosis, high relapse rates, and reduced overall survival. Patients with FLT3 mutation−positive AML, both ITD and TKD, could be eligible for FDA-approved targeted therapies such as midostaurin and gilteritinib.

Performing Laboratory
med fusion

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.