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Methicillin Resistant Staph Aureus, PCR : 17656
Test CodeMRSAPC or 17656
Alias/See Also
MRSA PCR, Staphylococcus aureus, Methicillin-resistant
CPT Codes
87641
Instructions
ESwab: When using the ESwab, collect the nasal specimen by sampling both nares one at a time with the same swab. Place the swab into the transport tube containing the Liquid Amies Transport Medium.
Rayon Dual nasal swabs: When using the dual rayon swabs, keep both swabs always attached to the red cap. Holding the swab cap with both swabs attached, sample each nare one at a time. Place the dual swab specimens into the transport tube containing the Liquid Stuart Medium or Liquid Amies Transport Medium.
Rayon Dual nasal swabs: When using the dual rayon swabs, keep both swabs always attached to the red cap. Holding the swab cap with both swabs attached, sample each nare one at a time. Place the dual swab specimens into the transport tube containing the Liquid Stuart Medium or Liquid Amies Transport Medium.
Transport Container
Preferred: Eswab (white polypropylene screw-cap tube with 1 mL of Liquid Amies medium)
Other Acceptable: Rayon Dual nasal swabs the transport tube containing the Liquid Stuart Medium or Liquid Amies Transport Medium.
Other Acceptable: Rayon Dual nasal swabs the transport tube containing the Liquid Stuart Medium or Liquid Amies Transport Medium.
Transport Temperature
Refrigerated.
Specimen Stability
Room temperature: 24 hours
Refrigerated: 7 days
Frozen: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen, Specimens exceeding stability, Specimens in leaking or broken containers, Non-validated specimen types.Wire swabs
Methodology
Polymerase Chain Reaction (PCR)
Setup Schedule
Sunday - Saturday
Report Available
1 day
Reference Range
NOT DETECTED
Clinical Significance
Staphylococcus aureus is a major nosocomial pathogen that causes a range of diseases including endocarditis, osteomyelitis, toxic shock syndrome, food poisoning, carbuncles and boils. Methicillin-resistant S. aureus (MRSA) strains were first identified in 1960’s and were related to acquisition of mecA gene. Nasal colonization with MRSA has been shown to be a significant risk factor for transmission of MRSA to other hospitalized or institutionalized patients. Colonization may also lead to self-infection. In the US today, MRSA is responsible for approximately 25-30% of nosocomial infections and reports of community-acquired MRSA are increasing, resulting in significant morbidity and mortality.
The rapid MRSA PCR is designed for direct detection of nasal MRSA colonization and is intended to aid in the prevention and control of MRSA infections in healthcare settings. Targets include the proprietary sequence of Staphylococcus chromosomal cassette (SCC) gene. Rapid MRSA PCR is not intended to guide or monitor treatment for MRSA infections. Concomitant cultures are necessary only to recover organisms for epidemiological typing or for further susceptibility testing.
The rapid MRSA PCR is designed for direct detection of nasal MRSA colonization and is intended to aid in the prevention and control of MRSA infections in healthcare settings. Targets include the proprietary sequence of Staphylococcus chromosomal cassette (SCC) gene. Rapid MRSA PCR is not intended to guide or monitor treatment for MRSA infections. Concomitant cultures are necessary only to recover organisms for epidemiological typing or for further susceptibility testing.
Performing Laboratory
med fusion
