Testosterone, Free, Bioavailable and Total, Males (Adult), Immunoassay : 30741

Test Code
TESSBC or 30741


CPT Codes
84403, 84270, 82040

Includes
Panel includes: Testosterone, Total, Males (Adult), Immunoassay; Albumin; Sex Hormone Binding Globulin (SHBG); Testosterone, Free and Bioavailable (calculated)


Instructions
Due to changes in testosterone levels throughout the day, two morning (8:00-10:00 a.m.) specimens obtained on different days are recommended by The Endocrine Society for screening.

Dietary supplements containing biotin may interfere in assays and may skew results to be either falsely high or falsely low. For patients receiving the recommended daily doses of biotin, draw samples at least 8 hours following the last biotin supplementation. For patients on mega-doses of biotin supplements, draw samples at least 72 hours following the last biotin supplementation.
   
Note: This assay should be performed on adult (≥18 years) males.


Transport Container
Preferred Specimen:
2 mL serum collected in a red-top tube (no gel)
     
Alternative Specimen:
Serum Separator Tube (SST)
 
Minimum Volume
1.5 mL


Transport Temperature
Room temperature.


Specimen Stability
Room temperature: 5 days; Refrigerated: 7 days; Frozen: 21 days


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Grossly icteric


Methodology
Immunoassay

Setup Schedule
Sunday - Saturday


Report Available
1 day


Reference Range
Testosterone, Total, Males (Adult), Immunoassay 
Male        250-827 ng/dL 
Female     Not applicable 
All test requests for Testosterone on female and pediatric (<18 years) patients must use test code 15983 - Testosterone, Total, MS.


Albumin      3.6-5.1 g/dL 

Sex Hormone Binding Globulin 
Age                  Male (nmol/L)         Female (nmol/L) 
<3 Years         Not established      Not established 
3-9 Years        32-158                     32-158 
10-13 Years    20-166                     24-120 
14-17 Years    20-87                        12-150 
18-55 Years    10-50                        17-124 
>55 Years        22-77                        14-73 

Free Testosterone 
Age                    Male (pg/mL)           Female 
18-69 Years    46.0-224.0                Not applicable 
>69 Years         6.0-73.0                    Not applicable 

Bioavailable Testosterone 
Age                      Male (ng/dL)           Female 
18-69 Years       110.0-575.0            Not applicable 
>69 Years            15.0-150.0             Not applicable 


Clinical Significance
Testosterone circulates almost entirely bound to transport proteins: Normally less than 1-2% is free. The principal transport protein for testosterone is known as sex hormone binding globulin (SHBG) or testosterone-estradiol binding globulin (TeBG). Testosterone measurements are used to assess erectile dysfunction, infertility, gynecomastia, and osteoporosis and to assess hormone replacement therapy.
  


Performing Laboratory
med fusion



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.