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QuestAssureD 25-Hydroxyvitamin D (D2, D3) : 92888
Test CodeVTD25 or 92888
Alias/See Also
Vitamin D2, 25-hydroxy,25-Hydroxyergocalciferol (25OHD2),Vitamin D3, 25-hydroxy,D2 Vitamin,25-Hydroxyvitamin D3,25-OH-D3,25-Hydroxycholecalciferol (25OHD3),25-OH-D2,D3 Vitamin,Vit D, LC/MS/MS,25-Hydroxyvitamin D2
CPT Codes
82306
Includes
Includes Vitamin D, 25-OH, Total; Vitamin D, 25-OH, D3; Vitamin D, 25-OH, D2
Instructions
Fasting preferred, but not required.
Collect blood in a standard red-top serum Vacutainer tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Alternatively, collect blood in a Serum Separator Tube (SST), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours.
Collect blood in a standard red-top serum Vacutainer tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Alternatively, collect blood in a Serum Separator Tube (SST), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours.
Transport Container
Specimen:
0.5 mL serum
Minimum Volume: 0.3 mL
0.5 mL serum
Minimum Volume: 0.3 mL
Transport Temperature
Room Temperature.
Specimen Stability
Room temperature:: 21 days; Refrigerated: 21 days; Frozen: 21 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis, Grossly lipemic, Heparinized or EDTA plasma, Serum not separated from Serum Separator Tube (SST) gel or clot within 48 hours, Grossly icteric
Methodology
Chromatography/Mass Spectrometry
Setup Schedule
Sunday - Saturday
Report Available
2-3 days
Limitations
This test was developed and its analytical performance
characteristics have been determined by medfusion. It has
not been cleared or approved by the US Food and Drug
Administration. This assay has been validated pursuant to
the CLIA regulation and is used for Clinical purposes.
characteristics have been determined by medfusion. It has
not been cleared or approved by the US Food and Drug
Administration. This assay has been validated pursuant to
the CLIA regulation and is used for Clinical purposes.
Reference Range
Vitamin D, 25-OH, Total 30-100 ng/mL
Vitamin D, 25-OH, D3 Not established
Vitamin D, 25-OH, D2 Not established
Vitamin D, 25-OH, D3 Not established
Vitamin D, 25-OH, D2 Not established
Clinical Significance
Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25-OH D3 - the endogenous form of the vitamin and 25-OH D2 - the analog form used to treat 25-OH Vitamin D3 deficiency.
Performing Laboratory
med fusion
