Vitamin D, 1,25-Dihydroxy : 16558

Test Code
VD125 or 16558

CPT Codes
82652

Includes
Includes Vitamin D,1,25 (OH)2,Total; Vitamin D3, 1,25 (OH)2; Vitamin D2, 1,25 (OH)2

Instructions
Collect blood in a non-gel barrier red-top tube. Allow blood to clot (30 minutes) at room temperature. Centrifuge and separate the serum from the cells.

Alternatively, collect blood in a Serum Separator Tube (SST), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours.

Note: If sample is submitted with less than 1.1 mL and needs to be repeated, the sample will be cancelled with the comment "TNP-Initial testing necessitated a repeat, but there was insufficient sample to perform repeat".



Note: If sample is submitted with less than 1.1 mL and needs to be repeated, the sample will be cancelled with the comment "TNP-Initial testing necessitated a repeat, but there was insufficient sample to perform repeat".

Transport Container
Specimen:
1.1 mL serum
Minimum Volume: 0.5 mL

Transport Temperature
Room temperature.

Specimen Stability
Room temperature: 28 days; Refrigerated: 28 days; Frozen: 28 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis, Lipemia, Icteric specimens, Plasma

Methodology
Chromatography/Mass Spectrometry

Setup Schedule
Sunday - Saturday

Report Available
2-3 days

Limitations
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range
Vitamin D, 1,25 (OH)2, Total   
1-9 Years           31-87 pg/mL 
10-13 Years       30-83 pg/mL 
14-17 Years       19-83 pg/mL 
Adult                  18-72 pg/mL 
Vitamin D3, 1,25 (OH)2 Not established 
Vitamin D2, 1,25 (OH)2 Not established 
Reference ranges are established for total 1,25-dihydroxy vitamin D. Values for subcomponents D2 (derived from plant or fungal sources) and D3 (derived from human or animal sources) are provided for informational purposes only.

Clinical Significance
This test measures the bioactive form of vitamin D. It is used in the differential diagnosis of hypocalcemia and to monitor patients with renal osteodystrophy or chronic renal failure. This test is not suitable for diagnosis of vitamin D deficiency and monitoring supplementation in most patients. The 25-hydroxyvitamin D test is the recommended test for those purposes 

Performing Laboratory
med fusion



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.