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HIV 1 RNA, Quantitative Real Time PCR : 40085
Test CodeHIVQNT or 40085
Alias/See Also
HIV Viral Load; Viral Load; HIV
CPT Codes
87536
Instructions
Do not freeze whole blood or any samples stored in primary tube. Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation. Follow manufacturer’s instructions for collection tube handling.
Primary tubes or aliquots from primary tubes preferred; specimen sharing is not recommended.
Primary tubes or aliquots from primary tubes preferred; specimen sharing is not recommended.
Transport Container
3 mL plasma collected in an EDTA (lavender-top) or PPT (white-top) tube
Minimum Volume: 1 mL
Minimum Volume: 1 mL
Transport Temperature
Frozen.
Specimen Stability
Room temperature: Unacceptable; Refrigerated: 6 days; Frozen: 84 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received room temperature; Plasma received frozen in the plasma preparation tube (PPT); Serum; Whole blood >24 hours old; Frozen whole blood
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Sunday - Saturday
Report Available
1-2 days
Reference Range
Reportable range: 20 to 10,000,000 copies/mL or 1.30 to 7.00 log copies/mL
Reference range:
Not detected copies/mL
Not detected log copies/mL
Reference range:
Not detected copies/mL
Not detected log copies/mL
Clinical Significance
This test is intended for use in conjunction with clinical presentation and other laboratory markers of disease progress for the clinical management of HIV-1 infected patients. The test can be used to assess patient prognosis by measuring the baseline HIV-1 RNA level or to monitor the effects of antiretroviral therapy by measuring changes in EDTA plasma HIV-1 RNA levels during the course of antiretroviral treatment.
Performing Laboratory
med fusion