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Direct Antiglobulin Test (DAT) with Reflex to Anti C3 and Anti IgG : 36668
MessageIf submitting with any other test requiring whole blood, please submit a separate whole blood tube for the DAT.
Test Code
QDAT or 36668
Alias/See Also
Coombs Direct; Direct Coombs
CPT Codes
86880
Includes
If DAT (Coombs, Direct) is positive, Anti C3d and Anti IgG will be performed at an additonal charge (CPT code(s): 86880x2).
Instructions
EDTA lavender or pink-top tube or ACD-A or ACD-B (yellow-top) tube.
Transport Container
Submit 5 mL (Min: 1 mL) whole blood tube.
Transport Temperature
Refrigerated.
Specimen Stability
Room temperature: 24 hours; Refrigerated: 72 hours; Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis, Grossly lipemic, Serum Separator tube, Clotted specimen, Grossly icteric.
Methodology
Agglutination
Setup Schedule
Monday - Friday
Report Available
1-4 days (From receipt at performing laboratory)
Reference Range
Negative
Clinical Significance
The DAT (Direct Coomb's test) is positive if red cells have been coated, in vivo, with immunoglobulin, complement, or both. A positive result can occur in immune-mediated red cell destruction, autoimmune hemolytic anemia, a transfusion reaction or in patients receiving certain drugs.
Performing Laboratory
Quest Diagnostics