A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Cryofibrinogen : 376
Test CodeCRFIBQ or 376
CPT Codes
82585
Instructions
Overnight fasting is required.
Collect blood into three 3.2% sodium citrate (light blue-top) tubes or three EDTA (lavender-top) tubes and place immediately in a 37° C. water bath, oven, incubator, or heat block or heel warmer(s) for 1 hour. It is very important that the specimen remain at 37° C until after separation of plasma from red cells.
Centrifuge the specimen for 10 minutes at 1600 RCF (G) or at top speed utilizing one of the 3 options below:
1. In a "warm" centrifuge at 37° C
2. In a bench-top centrifuge placed in a "warm room" at 37° C
3. At room temperature, in a bench-top centrifuge prewarmed by running it for 10 minutes at 1600 RCF (G) or at top speed.
After centrifuging, decant plasma into a screw-cap vial.
Transport at room temperature. Do not refrigerate or freeze.
Note: If the sample is NOT kept at 37° C during all processing steps, the cryoprecipitate if present can precipitate out of solution and be lost during the centrifugation process and result in a false negative result.
Do not refrigerate or freeze.
Note: No tests can be added or poured off this tube; for cryofibrinogen evaluation only.
Collect blood into three 3.2% sodium citrate (light blue-top) tubes or three EDTA (lavender-top) tubes and place immediately in a 37° C. water bath, oven, incubator, or heat block or heel warmer(s) for 1 hour. It is very important that the specimen remain at 37° C until after separation of plasma from red cells.
Centrifuge the specimen for 10 minutes at 1600 RCF (G) or at top speed utilizing one of the 3 options below:
1. In a "warm" centrifuge at 37° C
2. In a bench-top centrifuge placed in a "warm room" at 37° C
3. At room temperature, in a bench-top centrifuge prewarmed by running it for 10 minutes at 1600 RCF (G) or at top speed.
After centrifuging, decant plasma into a screw-cap vial.
Transport at room temperature. Do not refrigerate or freeze.
Note: If the sample is NOT kept at 37° C during all processing steps, the cryoprecipitate if present can precipitate out of solution and be lost during the centrifugation process and result in a false negative result.
Do not refrigerate or freeze.
Note: No tests can be added or poured off this tube; for cryofibrinogen evaluation only.
Transport Container
Preferred Specimen
6 mL fasting plasma collected in each of three 3.2% sodium citrate (light blue-top) tubes
Alternative Specimen
Fasting plasma collected in each of three EDTA (lavender-top) tubes
Minimum Volume
3 mL (x3)
6 mL fasting plasma collected in each of three 3.2% sodium citrate (light blue-top) tubes
Alternative Specimen
Fasting plasma collected in each of three EDTA (lavender-top) tubes
Minimum Volume
3 mL (x3)
Transport Temperature
Room temperature.
Specimen Stability
After separation from cells: Room temperature: 7 days; Refrigerated: Unacceptable; Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Heparinized plasma, Gross hemolysis, Grossly lipemic, Grossly icteric, Received refrigerated or frozen, <3 mL of plasma received
Methodology
Cold Precipitation
Setup Schedule
Sunday - Friday
Report Available
5-7 days (From receipt at performing laboratory)
Reference Range
Negative
Clinical Significance
Cryofibrinogens are cryoproteins that precipitate only in plasma and not in serum when exposed to temperatures below 37° C and dissolves upon rewarming. Cryofibrinogens can occur independently or in association with cryoglobulins. Cryofibrinogens are composed of fibrinogen, fibronectin and fibrin but may also contain albumin and/or immunoglobulins. Fibrin and fibronectin are needed to induce the formation of cryofibrinogen and cold precipitation. In isolated Cryofibrinogenemia, the precipitate rarely contains immunoglobulins. However, when cryofibrinogenemia, is associated with cryoglobulinemia, the characteristic components of Cryofibrinogen contain polyclonal immunoglobulins in 80% cases and show a pattern similar to cryoglobulins. Cryoprecipitates in the serum are considered as positive cryoglobulins and cryoprecipitates in the plasma are positive as the sum of cryoglobulins and cryofibrinogens, both tests should be always performed simultaneously for differentiation.
Performing Laboratory
Quest Diagnostics