HIV-1 Coreceptor Tropism, Ultradeep Sequencing : 94014

Test Code

HIVCOR or 94014

CPT Codes
87906

Instructions

Lavender (EDTA) tube.

Transport Container

Separate plasma from cells within 24 hours of collection by centrifugation. Transfer 2 mL (Min: 0.6 mL) plasma to a standard transport tube.

Transport Temperature

Refrigerated.

Specimen Stability

After separation from cells: Room temperature: 24 hours; Refrigerated: 5 days; Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis, Lipemia, Serum, Heparinized plasma, Whole blood greater than 24 hours old.

Methodology
Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), Ultradeep Sequencing

Setup Schedule

Monday & Thursday

Report Available

4-11 days (From receipt at performing laboratory)

Reference Range

See Laboratory Report.

Clinical Significance

The use of CCR5 antagonists in patients harboring CXCR4(X4) or dual-mixed (DM) viruses has proven to be ineffective and leads to the emergence of X4 viruses as the predominant species in DM patients. In clinical trials, X4 virus was found in 55% of patients failing maraviroc therapy vs. only 9% of patients who experienced treatment failure in the placebo arm. The use of CCR5 antagonists, therefore, requires screening for viral tropism to exclude patients harboring X4 or DM virus. Detection of X4 virus prior to the initiation of therapy has been associated with a reduced response to maraviroc.

Performing Laboratory
Quest Diagnostics

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.