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Herpes Simplex Virus Culture with Reflex Typing : 2649
Test CodeQHSVCR or 2649
Alias/See Also
Herpes Simplex Virus Culture with Reflex Typing
CPT Codes
87255
Includes
If culture is positive, typing will be performed at an additional charge (CPT code(s): 87140 per typing).
Instructions
Nasal/nasopharyngeal swab, endocervical swab, eye swab or lesion (vesicle) aspirate swab, urethral swab, vaginal swab, rectal mucosa swab or throat swab collected in V-C-M (green-cap) tube or FDA equivalent.
Alternative specimen: 3 mL bronchial lavage/wash or nasopharyngeal lavage/wash or Fresh (unfixed) tissue (1 g) collected in V-C-M (green-cap) tube or FDA equivalent or sterile screw-cap container.
Alternative specimen: 3 mL bronchial lavage/wash or nasopharyngeal lavage/wash or Fresh (unfixed) tissue (1 g) collected in V-C-M (green-cap) tube or FDA equivalent or sterile screw-cap container.
Transport Container
To maintain optimum viability, place swab into V-C-M (equal volumes of fluid/tissue and V-C-M) or equivalent and transport the specimen to the laboratory as soon as possible. Best recovery is obtained when the specimens are refrigerated at 2-8° C or kept on wet ice following collection and while in transit. If there will be a long delay before processing, specimens in V-C-M or equivalent should be frozen at -70° C or colder and transported on dry ice. Storage or transport at -20° C is not acceptable. Raw (unpreserved) samples should only be refrigerated and not frozen.
Transport Temperature
Refrigerated.
Specimen Stability
V-C-M or equivalent: Room temperature: Unacceptable; Refrigerated: 4 days; Frozen -20° C: Unacceptable; Frozen -70° C: 30 days
Raw (unpreserved) specimens: Room temperature: Unacceptable; Refrigerated: 72 hours; Frozen: Unacceptable
Raw (unpreserved) specimens: Room temperature: Unacceptable; Refrigerated: 72 hours; Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
CSF, Sputum, Raw (unpreserved) stool, Dry swabs, Molecular transport systems, Bacterial transport systems, Tissue or biopsies in formalin or other fixatives, Calcium alginate swabs, Wooden shaft swabs, Non gel-based bacterial transports.
Methodology
Centrifuge Enhanced Culture, Histochemical Stain, Monoclonal Fluorescent Antibody Typing
Setup Schedule
Tuesday - Saturday
Report Available
3-5 days (From receipt at performing laboratory)
Reference Range
Negative
Clinical Significance
A clinical diagnosis of HSV infection is based on identifying characteristic multiple vescicles on an erythematous base. HSV culture is useful in verifying HSV is present within the vesicles. Rapid culture allows for the initiation of therapy that may diminish the severity of symptoms and shorten the length of symptoms.
Performing Laboratory
Quest Diagnostics