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PDGFRB (5q32) gene rearrangement by FISH : 1003118
MessagePlease provide most recent Pathology report.
Test Code
MDFCPPDGFB or 1003118
Alias/See Also
platelet derived growth factor receptor beta
CPT Codes
88271x2, 88275
Instructions
Bone Marrow aspirate in a green top sodium heparin tube (2-3 mL) or Peripheral Blood in a green top sodium heparin tube (2-10 mL). Tissue also accepted.
Transport Container
Blood or Bone Marrow: Do not centrifuge. Paraffin embedded formalin fixed tissue that has been fixed in 10% neutral buffered formalin for at least 6 hours and no longer than 48 hours. Four unstained slides, with tissue of 4 microns in thickness are needed for processing, accompanied by a circled H & E clearly indicating the area to be examined.
Transport Temperature
Blood or Bone Marrow: Ambient temperature within 24 hours. Specimen can be refrigerated if not transported immediately. Do not freeze. Protect from heat with a cold pack. Paraffin embedded tissue block: Ambient or on ice pack in summer. Slides:
Ambient.
Ambient.
Specimen Stability
Blood and Bone Marrow: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Paraffin embedded tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Paraffin embedded tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Methodology
Fluorescence in situ hybridization (FISH)
Setup Schedule
Monday - Friday
Report Available
Up to 7 days
Limitations
Laboratory test results should always be considered in the context of clinical observations. This test was developed and its performance characteristics determined by med fusion. It has not been cleared or approved by the U.S. Food and Drug Administration
(FDA). The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments
of 1988 (CLIA) as qualified to perform high complexity clinical laboratory testing.
(FDA). The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments
of 1988 (CLIA) as qualified to perform high complexity clinical laboratory testing.
Reference Range
An interpretive report will be provided.
Clinical Significance
PDGFRB gene rearrangements have been associated with phenotypically and genotypically diverse types of neoplasms. These include chronic eosinophilic leukemia and neoplasms associated with eosiniphilia such as chronic myelomonocytic leukemia and atypical chronic myeloid leukemia. Genetically, PDGFRB gene is known to partner with more than 20 genes facilitated by different chromosomal rearrangements most often visualized as cytogenetically detectible translocations. These genetic alterations result in constitutive activation of the receptor tyrosine kinase, PDGFRB and respond to tyrosine kinase inhibitor therapy. Therefore, identifying the PDGFRB gene rearrangement status is crucial.
The PDGFRB FISH assay is designed to detect the PDGFRB gene rearrangement regardless of the knowledge of the partner gene involved and can be effectively performed in non-dividing cells
The PDGFRB FISH assay is designed to detect the PDGFRB gene rearrangement regardless of the knowledge of the partner gene involved and can be effectively performed in non-dividing cells
Performing Laboratory
med fusion
