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IGH-CCND1 (BCL-1/JH), Translocation, t(11;14) by PCR : 14991
Test CodeIGHCCN or 14991
Alias/See Also
BCL-1 (IGH-CCND1 (BCL-1/JH) Translocation, t(11;14) by PCR, Fluid); Mantle Cell Lymphoma (IGH-CCND1 (BCL-1/JH) Translocation, t(11;14) by PCR, Fluid); bcl-1/JH t(11;14) (BCL-1/JH) Translocation, t(11;14) by PCR, Fluid)
CPT Codes
81168
Instructions
Lavender (EDTA) OR bone marrow (EDTA) OR tissue .
Transport Container
Whole blood: Transport 5 mL. (Min: 1 mL) OR Bone marrow: Transport 3 mL. (Min: 1 mL) OR Fresh tissue: Freeze immediately or place in RPMI. Transport 100 mg or 0.5-2.0 cm3 tissue. OR FFPE tumor tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block. Tissue block will be returned after testing.
Transport Temperature
Whole blood OR Bone marrow: Refrigerated. Fresh tissue: Frozen on dry ice. Tissue in RPMI or formalin fixative: Refrigerated. FFPE tumor tissue: Ambient. Ship in cooled container during summer months.
Specimen Stability
Whole blood OR Bone marrow: Ambient: 7 days; Refrigerated: 14 days; Frozen: Unacceptable
Fresh Tissue : Ambient: Unacceptable; Refrigerated: 2 hours; Frozen: 1 year
Tissue in formalin or RPM!: Ambient: Indefinitley; Refrigerated: Indefinitely; Frozen: Unacceptable
FFPE tumor tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Fresh Tissue : Ambient: Unacceptable; Refrigerated: 2 hours; Frozen: 1 year
Tissue in formalin or RPM!: Ambient: Indefinitley; Refrigerated: Indefinitely; Frozen: Unacceptable
FFPE tumor tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Whole blood OR Bone marrow: Frozen specimens. Clotted or grossly hemolyzed specimens.
FFPE tumor tissue: Specimens fixed/processed in alternative fixatives or heavy metal fixatives (B-4 or B-5) or tissue sections on slides. Decalcified specimens.
FFPE tumor tissue: Specimens fixed/processed in alternative fixatives or heavy metal fixatives (B-4 or B-5) or tissue sections on slides. Decalcified specimens.
Methodology
Polymerase Chain Reaction
Setup Schedule
Tuesday & Saturday
Report Available
3-7 days (From receipt at performing laboratory)
Limitations
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Reference Range
0/Negative
Clinical Significance
Bcl-1/JH t(11;14) is highly specific for mantle cell lymphoma (MCL). The t(11;14) can be found in approximately 70% to 95% cases of MCL. Half of these translocations fall within the BCL-1 major translocation cluster (MTC). The t(11;14) Real-Time PCR assay can be used to monitor minimal residual disease in NHL.
Performing Laboratory
Quest Diagnostics
