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Anti-Nuclear Antibodies, IFA, with Reflex to Multiplex Antibody Comprehensive : 1003718
Test CodeANACOM or 1003718
Alias/See Also
Lupus Testing; ANA; Antinuclear Antibodies; Connective Tissue Disease; Autoantibody Testing
CPT Codes
86038
Includes
If the ANA by IFA is positive, a titer will be performed at an additional charge (CPT code(s): 86039). If the ANA by IFA is positive at a titer >=1:80, then the ENA panel will be performed at an additional charge. ENA panel includes: SSA, SSB, RNP, SMITH, Jo-1, Scl-70, ds DNA multiplex flow, Centromere, Chromatin, Ribosomal P, and SmRNP. (CPT code(s): 86225, 83516, 86235 x9).
Preferred Specimen
1 mL serum
Instructions
Serum separator tube (gold top preferred) or a plain red top tube.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 4 days
Refrigerated: 7 days;
Frozen: 30 days
Refrigerated: 7 days;
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis, grossly lipemic, microbial contamination may interfere
Methodology
Indirect Immunofluorescence; Multiplex Flow Immunoassay
Setup Schedule
Monday - Saturday
Report Available
1-3 days
Reference Range
ANA Screen, IFA: Negative
ANA Titer: <1:80
ANA Pattern: None
ANA Titer 2: <1:80
ANA Pattern 2: None
If reflex to ENA then all analytes Negative with the exception of dsDNA.
dsDNA :
Negative <or = 4 IU/mL
Indeterminate 5-9 IU/mL
Positive > or = 10 IU/mL
ANA Titer: <1:80
ANA Pattern: None
ANA Titer 2: <1:80
ANA Pattern 2: None
If reflex to ENA then all analytes Negative with the exception of dsDNA.
dsDNA :
Negative <or = 4 IU/mL
Indeterminate 5-9 IU/mL
Positive > or = 10 IU/mL
Clinical Significance
Used as an aid in the diagnosis of systemic autoimmune diseases.
Performing Laboratory
med fusion
