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Bartonella Species Antibodies (IgG, IgM) with Reflex to Titer : 34251
Test CodeBARTRQ or 34251
CPT Codes
86611x4
Includes
Includes: Bartonella henselae IgG, IgM; Bartonella quintana IgG, IgM.
If screen is positive, then titers will be performed at an additional charge (CPT code(s): 86611 per titer). (If reflex: non-orderables %38514, %38516, %38518, or %38520)
If screen is positive, then titers will be performed at an additional charge (CPT code(s): 86611 per titer). (If reflex: non-orderables %38514, %38516, %38518, or %38520)
Instructions
Serum separator tube or plain red.
Transport Container
Centrifuge the serum separator tube and transport. If plain red, centrifuge and aliquot 1 mL serum (Min: 0.2 mL) into a standard transport tube.
Transport Temperature
Refrigerated.
Specimen Stability
Ambient: 7 days; Refrigerated: 14 days; Frozen: 30 days
Methodology
Immunofluorescence Assay (IFA)
Setup Schedule
3 days per week
Report Available
1-4 days (From receipt at performing laboratory)
Limitations
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Reference Range
Negative
Clinical Significance
This antibody panel helps diagnose infections with Bartonella henselae and Bartonella quintana. This panel includes IgM and IgG antibodies with reflexes to titers for positive results.
B henselae causes cat scratch disease, a self-limiting bacterial infection transmitted via exposure to cats or cat fleas. B quintana causes trench fever, a febrile bacteremic illness transmitted via body lice. B henselae and B quintana are the 2 Bartonella species that most frequently cause blood culture-negative endocarditis. In immunocompromised individuals, infections with B henselae and B quintana may present as bacillary angiomatosis, a vasculoproliferative disease of the skin [1-4].
Because of the fastidious nature of B henselae and B quintana, recovery of these bacteria is rarely successful, especially from blood. Serology is commonly used for diagnosing Bartonella infections [1,3]. Confirmation of recent or current infection with either B henselae or B quintana may require testing of serial specimens to demonstrate a 4-fold increase of IgG titers or the presence of IgM. A B henselae or B quintana IgG titer of 1:800 has been proposed as a major criterion for diagnosing Bartonella endocarditis [4].
IgG cross-reactivity between B henselae and B quintana often occurs [1,3]. Qualitative real-time polymerase chain reaction is a highly specific and sensitive method to detect the presence of Bartonella species DNA in clinical specimens and can differentiate between B henselae and B quintana.
The results of this test should be interpreted in the context of pertinent clinical history and physical examination findings.
References
1. Dumler JS, et al. Bartonella. In: Carroll KC, et al, eds. Manual of Clinical Microbiology. 12th ed. ASM Press; 2019. https://www.clinmicronow.org/doi/book/10.1128/9781683670438.MCM.ch50
2. Centers for Disease Control and Prevention. Bartonella infection (cat scratch disease, trench fever, and Carrión’s disease). Last reviewed December 18, 2019. Accessed January 28, 2022. https://www.cdc.gov/bartonella/clinicians/index.html
3. Miller JM, et al. Clin Infect Dis. 2018;67(6):813-816.
4. Okaro U, et al. Clin Microbiol Rev. 2017;30(3):709-746.
B henselae causes cat scratch disease, a self-limiting bacterial infection transmitted via exposure to cats or cat fleas. B quintana causes trench fever, a febrile bacteremic illness transmitted via body lice. B henselae and B quintana are the 2 Bartonella species that most frequently cause blood culture-negative endocarditis. In immunocompromised individuals, infections with B henselae and B quintana may present as bacillary angiomatosis, a vasculoproliferative disease of the skin [1-4].
Because of the fastidious nature of B henselae and B quintana, recovery of these bacteria is rarely successful, especially from blood. Serology is commonly used for diagnosing Bartonella infections [1,3]. Confirmation of recent or current infection with either B henselae or B quintana may require testing of serial specimens to demonstrate a 4-fold increase of IgG titers or the presence of IgM. A B henselae or B quintana IgG titer of 1:800 has been proposed as a major criterion for diagnosing Bartonella endocarditis [4].
IgG cross-reactivity between B henselae and B quintana often occurs [1,3]. Qualitative real-time polymerase chain reaction is a highly specific and sensitive method to detect the presence of Bartonella species DNA in clinical specimens and can differentiate between B henselae and B quintana.
The results of this test should be interpreted in the context of pertinent clinical history and physical examination findings.
References
1. Dumler JS, et al. Bartonella. In: Carroll KC, et al, eds. Manual of Clinical Microbiology. 12th ed. ASM Press; 2019. https://www.clinmicronow.org/doi/book/10.1128/9781683670438.MCM.ch50
2. Centers for Disease Control and Prevention. Bartonella infection (cat scratch disease, trench fever, and Carrión’s disease). Last reviewed December 18, 2019. Accessed January 28, 2022. https://www.cdc.gov/bartonella/clinicians/index.html
3. Miller JM, et al. Clin Infect Dis. 2018;67(6):813-816.
4. Okaro U, et al. Clin Microbiol Rev. 2017;30(3):709-746.
Performing Laboratory
Quest Diagnostics
