ADAMTS13 Activity with Reflex to Inhibitor : 1002426

Message
CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Test Code
ADAMST or 1002426

Alias/See Also
VWF Cleaving Protease (vWF-CP); vWF Protease Activity (ADAMTS-13); VWF Cleaving Protease (ADAMTS-13; vWF-CP (ADAMTS-13); von Willebrand Factor Protease Activity (ADAMTS-13)

CPT Codes
85397

Includes
If ADAMTS13 Activity is less than or equal to 30, ADAMTS13 Inhibitor will be performed at an additional charge (CPT code(s); 85335). (non-orderable 1016591)

Instructions
Lt. blue (3.2% sodium citrate).

Transport Container
Centrifuge light blue-top tube 15 minutes at approx. 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into standard plastic transport tube. Plasma must be free of platelets (less than 10,000/mcl). Freeze immediately 1 mL plasma (Min. 0.5 mL).

Transport Temperature
Frozen.

Specimen Stability
Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen -20 C: 21 days; Frozen -70 C: 6 months

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
EDTA plasma

Methodology
Immunoassay (IA)

Setup Schedule
Sunday - Thursday

Report Available
2-4 days (From receipt at performing laboratory)

Limitations
This test was performed using a kit that has not been cleared or approved by the FDA. The analytical performance characteristics of this test have been determined by Quest Diagnostics. This test should not be used for diagnosis without confirmation by other medically established means.

Reference Range
ADAMTS13 Activity 68-163 % Activity
If reflex: ADAMTS13 Inhibitor less than 0.4 BEU

Clinical Significance
ADAMTS-13 is a zinc metalloprotease that cleaves ultra large vWF multimers. Studies have shown that low levels of ADAMTS-13 activity are associated with thrombotic thrombocytopenic purpura (TTP), a life-threatening hematological condition characterized by a low platelet count, microvascular thrombi, red cell fragmentation, and renal complications. Congenital TTP is a rare inherited disease caused by mutations within the ADAMTS-13 gene, which result in the production of non-functional protein. The acquired form of TTP is an autoimmune-like disorder caused by the development of autoantibodies to ADAMTS-13 that inhibits enzyme activity.

Performing Laboratory
Quest Diagnostics



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.