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Toxoplasma gondii DNA, Qualitative Real-Time PCR : 34451
Test CodeTOXQL or 34451
Alias/See Also
T gondii DNA, Toxoplasmosis
CPT Codes
87798
Instructions
Amniotic fluid or CSF or tissue collected in a sterile screw cap container. Plasma collected in: EDTA (lavender-top), ACD (yellow-top) tube, SST (red-top) tube, Whole blood collected in: EDTA (lavender-top), ACD (yellow-top) tube, Vitreous fluid collected in a sterile screw cap container, Amniotic fluid supernatant collected in a sterile screw cap container. Do not dilute vitreous fluid with additional saline.
Transport Container
Transport 1 mL (0.3 mL minimum) serum collected in a red top tube (no gel) or Serum Separator Tube;
1 mL (0.3 mL minimum) Whole blood or plasma collected in: EDTA (lavender-top);
1 mL (0.3 mL minimum) Amniotic fluid supernatant collected in: Sterile leak-proof container;
1 mL (0.2 mL minimum) vitreous fluid collected in: Sterile leak-proof container;
3 cubic mm tissue collected in a sterile leak-proof container
1 mL (0.3 mL minimum) Whole blood or plasma collected in: EDTA (lavender-top);
1 mL (0.3 mL minimum) Amniotic fluid supernatant collected in: Sterile leak-proof container;
1 mL (0.2 mL minimum) vitreous fluid collected in: Sterile leak-proof container;
3 cubic mm tissue collected in a sterile leak-proof container
Transport Temperature
Refrigerated
Specimen Stability
Amniotic fluid, CSF, Tissue, Plasma, Serum, Vitreous fluid, or Amniotic fluid supernatant:
Room temperature: 48 hours; Refrigerated: 7 days; Frozen: 30 days
Whole blood: Room temperature: 48 hours; Refrigerated: 7 days; Frozen: Unacceptable
Room temperature: 48 hours; Refrigerated: 7 days; Frozen: 30 days
Whole blood: Room temperature: 48 hours; Refrigerated: 7 days; Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed whole blood
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Mon-Wed-Fri
Report Available
2-4 days
Limitations
Laboratory test results should always be considered in the context of clinical observations. This test was developed, and its performance characteristics determined by med fusion. It has not been cleared or approved by the U.S. Food and Drug Administration (FDA). The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA) as qualified to perform high complexity clinical laboratory testing.
Reference Range
Not Detected
Clinical Significance
Toxoplasma gondii, an obligate intracellular parasite, is an important opportunistic pathogen of immunosuppressed patients. In AIDS patients and transplant patients, this infection may result in a life-threatening encephalitis. T. gondii can also cause a fatal infection of the fetus if an infection is acquired during pregnancy. Fetal death or major abnormalities such as blindness and mental retardation may occur when infection is acquired during the first trimester. PCR methods may be useful in identifying T. gondii in CSF of immunosuppressed patients or in the amniotic fluid of mothers thought to be recently infected.
Performing Laboratory
med fusion
