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Toxoplasma gondii DNA, Qualitative Real-Time PCR : 34451
Test CodeTOXGOQ or 34451
Alias/See Also
T gondii DNA, Toxoplasmosis
CPT Codes
87798
Instructions
Amniotic fluid or CSF or tissue collected in a sterile screw cap container. Plasma collected in: EDTA (lavender-top), ACD (yellow-top) tube, SST (red-top) tube, Whole blood collected in: EDTA (lavender-top), ACD (yellow-top) tube, Vitreous fluid collected in a sterile screw cap container, Amniotic fluid supernatant collected in a sterile screw cap container. Do not dilute vitreous fluid with additional saline.
Transport Container
Transport 1 mL (Min: 0.3 mL) amniotic fluid or CSF, 3 cubic mm tissue (Min: 3 cubic mm), or 0.2 mL (Min: 0.2 mL) vitreous fluid. If sending plasma or serum or amniotic fluid supernatant, centrifuge and transfer to a standard transport tube. (Min: 0.3 mL). Transport whole blood.
Transport Temperature
Refrigerated.
Specimen Stability
Amniotic fluid, CSF, Tissue, Plasma, Serum, Vitreous fluid, or Amniotic fluid supernatant: Ambient: 48 hours; Refrigerated: 7 days; Frozen: 30 days
Whole blood: Ambient: 48 hours; Refrigerated: 7 days; Frozen: Unacceptable
Whole blood: Ambient: 48 hours; Refrigerated: 7 days; Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed whole blood.
Methodology
Real-Time PCR
Setup Schedule
Sunday - Saturday
Report Available
1-2 days (From receipt at performing laboratory)
Limitations
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Reference Range
Not detected.
Clinical Significance
Toxoplasma gondii, an obligate intracellular parasite, is an important opportunistic pathogen of immunosuppressed patients. In AIDS patients and transplant patients, this infection may result in a life-threatening encephalitis. T. gondii can also cause a fatal infection of the fetus if an infection is acquired during pregnancy. Fetal death or major abnormalities such as blindness and mental retardation may occur when infection is acquired during the first trimester. PCR methods may be useful in identifying T. gondii in CSF of immunosuppressed patients or in the amniotic fluid of mothers thought to be recently infected.
Performing Laboratory
Quest Diagnostics