Hemoglobin, Free, Plasma : 514

Message

Delayed separation from cells will falsely elevate plasma hemoglobin due to artifactual post draw RBC lysis.

Test Code

PHGBN or 514

CPT Codes
83051

Instructions

Green (no gel) (sodium or lithium heparin) or green plasma separator tube (lithium heparin).

Transport Container

Centrifuge green top and aliquot within 1 hour of collection 1 mL plasma (Min: 0.2 mL) into a standard transport tube. If green plasma separator tube, centrifuge within 1 hour of collection and transport.

Transport Temperature

Refrigerated.

Specimen Stability

Ambient: 7 days; Refrigerated: 7 days; Frozen: Unacceptable

Methodology
Colorimetry ( C)

Setup Schedule

Tuesday - Saturday

Report Available

1-2 days (From receipt at performing laboratory)

Limitations

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range

less than 8.4 mg/dL

Clinical Significance

The presence of free hemoglobin in plasma is an indication of intravascular hemolysis resulting from numerous conditions, among which include paroxysmal nocturnal hemoglobinuria (PNH), sickle-cell disease (SCD), thalassemias, hereditary spherocytosis, microangiopathic hemolytic anemias, pyruvate kinase deficiency, ABO mismatch transfusion reaction, cardiopulmonary bypass or mechanical heart valve-induced anemia.
Reference: JAMA. 2005;293(13):1653-1662

Performing Laboratory
Quest Diagnostics

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.