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Hemoglobin, Free, Plasma : 514
MessageDelayed separation from cells will falsely elevate plasma hemoglobin due to artifactual post draw RBC lysis.
Test Code
PHGBN or 514
CPT Codes
83051
Instructions
Green (no gel) (sodium or lithium heparin) or green plasma separator tube (lithium heparin).
Transport Container
Centrifuge green top and aliquot within 1 hour of collection 1 mL plasma (Min: 0.2 mL) into a standard transport tube. If green plasma separator tube, centrifuge within 1 hour of collection and transport.
Transport Temperature
Refrigerated.
Specimen Stability
Ambient: 7 days; Refrigerated: 7 days; Frozen: Unacceptable
Methodology
Colorimetry ( C)
Setup Schedule
Tuesday - Saturday
Report Available
1-2 days (From receipt at performing laboratory)
Reference Range
less than 8.4 mg/dL
Clinical Significance
The presence of free hemoglobin in plasma is an indication of intravascular hemolysis resulting from numerous conditions, among which include paroxysmal nocturnal hemoglobinuria (PNH), sickle-cell disease (SCD), thalassemias, hereditary spherocytosis, microangiopathic hemolytic anemias, pyruvate kinase deficiency, ABO mismatch transfusion reaction, cardiopulmonary bypass or mechanical heart valve-induced anemia.
Reference: JAMA. 2005;293(13):1653-1662
Reference: JAMA. 2005;293(13):1653-1662
Performing Laboratory
Quest Diagnostics