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Nicotine and Cotinine, Serum/Plasma : 90642
Test CodeNICOT or 90642
CPT Codes
80323 (HCPCS: G0480)
Instructions
Plain red or lavender (EDTA).
Transport Container
Centrifuge plain red or lavender top and aliquot 1 mL serum or plasma (Min: 0.5 mL) into a standard transport tube. Also acceptable: transport EDTA whole blood.
Transport Temperature
Refrigerated.
Specimen Stability
After separation from cells: Ambient: 72 hours; Refrigerated: 21 days; Frozen: 30 days
Whole blood: Ambient: 72 hours; Refrigerated: 21 days; Frozen: Unacceptable
Whole blood: Ambient: 72 hours; Refrigerated: 21 days; Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis; Serum Separator tube
Methodology
Liquid Chromatography/Mass Spectrometry
Setup Schedule
Monday & Wednesday & Friday
Report Available
4 days (From receipt at performing laboratory)
Limitations
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Reference Range
Nicotine
Smokers 2-10 ng/mL
Nonsmokers less than or equal to 4 ng/mL
Cotinine
Smokers 16-145 ng/mL
Nonsmokers less than or equal to 8 ng/mL
Smokers 2-10 ng/mL
Nonsmokers less than or equal to 4 ng/mL
Cotinine
Smokers 16-145 ng/mL
Nonsmokers less than or equal to 8 ng/mL
Clinical Significance
This assay is used for the detection of nicotine and cotinine in serum and plasma to determine the tobacco exposure status of the individual. Nicotine has a short half-life of approximately forty minutes; Its presence may indicate recent tobacco exposure. Cotinine, the major nicotine metabolite, has a half-life of 24 hours and is detectable for several days after cessation of tobacco exposure.
Performing Laboratory
Quest Diagnostics
