Nicotine and Cotinine, Serum/Plasma : 90642

Test Code
NICOT or 90642

CPT Codes

Plain red or lavender (EDTA).

Transport Container
Centrifuge plain red or lavender top and aliquot 1 mL serum or plasma (Min: 0.5 mL) into a standard transport tube. Also acceptable: transport EDTA whole blood.

Transport Temperature

Specimen Stability
After separation from cells: Ambient: 72 hours; Refrigerated: 21 days; Frozen: 30 days

Whole blood: Ambient: 72 hours; Refrigerated: 21 days; Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis; Serum Separator tube

Liquid Chromatography/Mass Spectrometry

Setup Schedule
Monday & Wednesday & Friday

Report Available
4 days (From receipt at performing laboratory)

Reference Range
Smokers 2-10 ng/mL
Nonsmokers less than or equal to 4 ng/mL

Smokers 16-145 ng/mL
Nonsmokers less than or equal to 8 ng/mL

Clinical Significance
This assay is used for the detection of nicotine and cotinine in serum and plasma to determine the tobacco exposure status of the individual. Nicotine has a short half-life of approximately forty minutes; Its presence may indicate recent tobacco exposure. Cotinine, the major nicotine metabolite, has a half-life of 24 hours and is detectable for several days after cessation of tobacco exposure.

Performing Laboratory
Quest Diagnostics

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.