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Bordetella pertussis/parapertussis DNA, Qualitative, PCR : 11365
Test CodeBPPPCR or 11365
Alias/See Also
Whooping cough
CPT Codes
87798x2
Instructions
Nasopharyngeal aspirate collected in a sterile vial OR collect nasopharyngeal swab.
Nasopharyngeal aspirates: Instill 1-1.5 mL of nonbacteriostatic saline (pH 7.0) into one nostril. Flush a plastic catheter or tubing with 2-3 mL of saline. Insert the tubing into the nostril parallel to the palate. Aspirate nasopharyngeal secretions. Repeat this procedure for the other nostril. Combine aspirates into a sterile vial.
Nasopharyngeal swab: Collection kits using Amies liquid, M4, UTM are acceptable. Swab tips may be polyester, rayon, nylon flocked. Flexible shafts must be used for proper collection. Accuracy of the test depends on the collection of ciliated epithelial cells material. Half the distance from the nostril to the base of the ear could be used as estimation of the site of collection. Gently insert swab into the nostril. Keep the swab near the septum floor of the nose while gently pushing into the posterior nasopharynx. Rotate swab 2-3 times and hold in place for few seconds for optimal collection. Collection of both nostrils is recommended but not required.
Nasopharyngeal aspirates: Instill 1-1.5 mL of nonbacteriostatic saline (pH 7.0) into one nostril. Flush a plastic catheter or tubing with 2-3 mL of saline. Insert the tubing into the nostril parallel to the palate. Aspirate nasopharyngeal secretions. Repeat this procedure for the other nostril. Combine aspirates into a sterile vial.
Nasopharyngeal swab: Collection kits using Amies liquid, M4, UTM are acceptable. Swab tips may be polyester, rayon, nylon flocked. Flexible shafts must be used for proper collection. Accuracy of the test depends on the collection of ciliated epithelial cells material. Half the distance from the nostril to the base of the ear could be used as estimation of the site of collection. Gently insert swab into the nostril. Keep the swab near the septum floor of the nose while gently pushing into the posterior nasopharynx. Rotate swab 2-3 times and hold in place for few seconds for optimal collection. Collection of both nostrils is recommended but not required.
Transport Container
Transport Nasopharyngeal Swab in viral transport media (M4. M5, VTM, or UTM) or in a Liquid Amies collection tube or eSwab (Liquid amies) or 1 mL (Min: 0.3 mL) Nasopharyngeal aspirate in a sterile container.
Transport Temperature
Refrigerated.
Specimen Stability
Nasopharyngeal aspirate:
Ambient: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Naspharyngeal swab:
Ambient: 7 days; Refrigerated: 7 days; Frozen: 30 days
Ambient: 48 hours; Refrigerated: 8 days; Frozen: 30 days
Naspharyngeal swab:
Ambient: 7 days; Refrigerated: 7 days; Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Collection tubes with Amies gel or charcoal containing media; Calcium-alginate swabs (shown to inhibit PCR); Rigid shaft swabs.
Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
Setup Schedule
Sunday - Saturday
Report Available
1-2 days (From receipt at performing laboratory)
Limitations
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Reference Range
B. pertussis DNA: Not detected
B. parapertussis DNA: Not detected
B. parapertussis DNA: Not detected
Clinical Significance
Bordetella pertussis is the cause of whooping cough that may occur in unimmunized individuals. B. parapertussis is a related organism that causes a similar but milder disease. Laboratory diagnosis may require both culture and serological confirmation although culture is difficult.
Performing Laboratory
Quest Diagnostics
