Multiple Myeloma Panel by Fish : 1001101

Test Code
MDFCPCMMP or 1001101

Alias/See Also
mm panel

CPT Codes
88271x11, 88275x5

Includes
Panel includes: D13S319 (13q13.4)/ATM (11q22.3)/IGH(14q32.3)/p53(17p13)/IGH-CCND1 (11q13)/ Aneuploidy 5,9,15/1q gain. Reflex: IGH-FGFR3, t(4;14))/IGH-MAF.

If IGH positive, then IGH/FGFR3 translocation, t(4;14) and IGH/MAF translocation, t(14,16) will be performed at an additional charge (CPT code(s): 88271x4, 88275x2).

Instructions
Bone Marrow aspirate in a green top sodium heparin tube (2-3 mL) or Peripheral Blood in a green top sodium heparin tube (2-10 mL).
    
     

Note: Bone Marrow is the preferred specimen and is used for plasma cell enrichment (using CD138 marker). Peripheral Blood is acceptable only if evidence of presence of plasma cells within the peripheral blood through Flow Cytometry studies or morphological studies is provided with the order.




Transport Container
Blood or Bone Marrow: Do not centrifuge.

Transport Temperature
Blood or Bone Marrow: Ambient temperature within 24 hours. Specimen can be refrigerated if not transported immediately. Do not freeze. Protect from heat with a cold pack.

Specimen Stability
Blood and Bone Marrow: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable

Methodology
Fluorescence in situ hybridization (FISH)

Setup Schedule
Monday - Friday

Report Available
Up to 7 days

Limitations
Laboratory test results should always be considered in the context of clinical observations. This test was developed and its performance characteristics determined by med fusion. It has not been cleared or approved by the U.S. Food and Drug Administration
(FDA). The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes. It should not be regarded as investigational for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments
of 1988 (CLIA) as qualified to perform high complexity clinical laboratory testing.

Reference Range
An interpretive report will be provided.

Clinical Significance
Conventional cytogenetics can detect abnormalities in about a third of multiple myeloma cases; FISH significantly increases the abnormality detection rate. Frequent translocation involving the heavy chain IGH on 14q32 with other partner genes such as Cyclin D1, C-MAF and FGFR3 can be easily detected by FISH. Translocations of IGH/FGFR3 and IGH/C-MAF and deletion of 17p/TP53 are considered as the most important independent negative prognostic indicator.

Performing Laboratory
med fusion



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.