Anti-Nuclear Antibodies, IFA, with Reflex to Multiplex Antibody Cascade

Test Code

ANACAS or 1003672

Alias/See Also

Autoantibody Testing; ANA; SSA; SSB; RNP; SMITH; Jo-1; Scl-70; Centromere; Chromatin; Ribosomal P; SmRNP; ds DNA; dsDNA

CPT Codes
86038

Includes

Cascades to Stage 1 if ANA is positive: Chromatin, ds DNA multiplex flow, RNP, SMITH, Sm RNP; if Stage 1 antibodies are negative then cascades to Stage 2: Jo-1, Scl-70, SSA, SSB; if Stage 2 antibodies are negative then cascades to Stage 3: Centromere, Ribosomal P antibodies. Each Stage of the Cascade is performed at an additonal charge (if reflex to Stage 1 then add CPT code(s) 86235x4, 86225, if reflex to Stage 2 then add CPT code(s): 86235x4, if reflex to Stage 3 then add CPT code(s): 86235, 83516.) (If reflex to Cascade, non-orderable code ANASB 1004264 added will be added.)

Preferred Specimen

1.5 mL serum

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 4 days
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis, grossly lipemic, microbial contamination may interfere

Methodology
Indirect Immunofluorescence

Setup Schedule

Monday - Saturday

Report Available

1-3 days

Reference Range

ANA Screen, IFA: Negative
ANA Titer: <1:80
ANA Pattern: None
ANA Titer 2: <1:80
ANA Pattern 2: None

Clinical Significance

Used as an aid in the diagnosis of systemic autoimmune diseases.

Performing Laboratory
med fusion

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.